Fabrication of a Chitosan-Coated Magnetic Nanobiocatalyst for Starch Hydrolysis

• Published in: Chemical engineering & technology Vol. 38; no. 8; pp. 1444 - 1451
• Main Authors: Jain, Manisha, Sebatini, Mariya, Sharmila, Govindasamy, Muthukumaran, Chandrasekaran, Baskar, Gurunathan, Tamilarasan, Krishnamurthi
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 01.08.2015; WILEY‐VCH Verlag
• Subjects: Amylase; Chitosan; Covalent immobilization; Hydrolysis; Immobilization; Magnetic nanoparticles; Nanoparticles; Nanostructure; Response surface methodology; Stability; Starch hydrolysis; Starches
• ISSN: 0930-7516; 1521-4125
• DOI: 10.1002/ceat.201400493

The preparation of chitosan‐coated magnetic nanoparticles (MNPs) and covalent immobilization of α‐amylase for starch hydrolysis was investigated. Surface morphology, chemical composition, and structural characteristics of the MNPs were analyzed by scanning electron microscopy, energy dispersion spectrometry, and X‐ray diffractometry, respectively. Surface functional groups of MNPs, chitosan‐coated MNPs, and α‐amylase‐immobilized MNPs were characterized by Fourier transform infrared spectroscopy. Response surface methodology based on three levels was implemented to optimize three immobilization conditions and a regression model was developed. α‐Amylase‐immobilized MNPs provided better stability towards pH and temperature. The prepared thermostable nanobiocatalyst is well‐suited for industrial processes involving starch hydrolysis. Magnetic nanoparticles (MNPs) can serve as a superior solid matrix for enzyme immobilization. Covalent immobilization of α‐amylase onto chitosan‐coated MNPs was optimized by response surface methodology. This nanobiocatalyst with high temperature and pH stability can be applied in industries for economical and highly efficient starch hydrolysis.