Salmon and human calcitonin-like peptides in man

Calcitonin-like peptides have been identified in the serum of normal subjects and of medullary thyroid carcinoma (MTC) patients. Using specific homologous radioimmunoassays (RIA) in combination with reversed-phase high performance liquid chromatography and gel permeation chromatography under denatur...

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Published inClinical endocrinology (Oxford) Vol. 20; no. 3; p. 253
Main Authors Tobler, P H, Tschopp, F A, Dambacher, M A, Fischer, J A
Format Journal Article
LanguageEnglish
Published England 01.03.1984
Subjects
Calcitonin - blood
Carcinoma - blood
Chromatography, Gel
Chromatography, High Pressure Liquid
Humans
Peptides - blood
Polydeoxyribonucleotides - blood
Radioimmunoassay
Thyroid Neoplasms - blood
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Summary:Calcitonin-like peptides have been identified in the serum of normal subjects and of medullary thyroid carcinoma (MTC) patients. Using specific homologous radioimmunoassays (RIA) in combination with reversed-phase high performance liquid chromatography and gel permeation chromatography under denaturing conditions, we have recognized major components which coeluted with human calcitonin-(1-32), PDN-21, a carboxyl-terminal flanking peptide derived from the calcitonin mRNA sequence, and salmon calcitonin-(1-32). An additional 12000 molecular weight peak possibly represents a human calcitonin-PDN-21 polyprotein. In both the human calcitonin-(1-32) (normal value less than 0.043 ngEq/ml; MTC 140 +/- 80 ngEq/ml, mean value +/- SEM) and the PDN-21 (normal value less than 0.050 ngEq/ml; MTC 33.6 +/- 16.5 ngEq/ml) RIAs, serum levels were increased in MTC patients. Circulating levels of the salmon calcitonin-like peptide were indistinguishable between normal subjects (0.038 +/- 0.006 ngEq/ml) and MTC patients (0.037 +/- 0.011 ngEq/ml).
ISSN:0300-0664
DOI:10.1111/j.1365-2265.1984.tb00081.x