Sample preparation procedures for biological atomic force microscopy

Summary Since the late 1980s, atomic force microscopy (AFM) has been increasingly used in biological sciences and it is now established as a versatile tool to address the structure, properties and functions of biological specimens. AFM is unique in that it provides three‐dimensional images of biolog...

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Bibliographic Details
Published inJournal of microscopy (Oxford) Vol. 218; no. 3; pp. 199 - 207
Main Authors EL KIRAT, K., BURTON, I., DUPRES, V., DUFRENE, Y. F.
Format Journal Article
LanguageEnglish
Published Oxford, UK Blackwell Publishing, Ltd 01.06.2005
Wiley
Subjects
Animals
Atomic force microscopy
Biochemistry
Biochemistry, Molecular Biology
biological samples
Cells
DNA
imaging
immobilization
Life Sciences
Lipids
Microscopy, Atomic Force - methods
Proteins
Specimen Handling - methods
substrates
substrates
Atomic force microscopy
immobilization
imaging
biological samples
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Summary:Summary Since the late 1980s, atomic force microscopy (AFM) has been increasingly used in biological sciences and it is now established as a versatile tool to address the structure, properties and functions of biological specimens. AFM is unique in that it provides three‐dimensional images of biological structures, including biomolecules, lipid films, 2D protein crystals and cells, under physiological conditions and with unprecedented resolution. A crucial prerequisite for successful, reliable biological AFM is that the samples need to be well attached to a solid substrate using appropriate, nondestructive methods. In this review, we discuss common techniques for immobilizing biological specimens for AFM studies.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-3
content type line 23
ObjectType-Review-1
ISSN:0022-2720
1365-2818
DOI:10.1111/j.1365-2818.2005.01480.x