HIV-1 low-level viremia affects T cell activation rather than T cell development in school-age children, adolescents, and young adults during antiretroviral therapy

• Published in: International journal of infectious diseases Vol. 91; pp. 210 - 217
• Main Authors: Han, Junyan, Mu, Weiwei, Zhao, Hongxin, Hao, Yu, Song, Chuan, Zhou, Haiwei, Sun, Xin, Li, Guoli, Dai, Guorui, Zhang, Yu, Zhang, Fujie, Zeng, Hui
• Format: Journal Article
• Language: English
• Published: Canada Elsevier Ltd 01.02.2020; Elsevier
• Subjects: Adolescent; Anti-HIV Agents - therapeutic use; Antiretroviral therapy; CD4 Lymphocyte Count; Child; Cross-Sectional Studies; Female; HIV Infections - drug therapy; HIV Infections - immunology; HIV Infections - virology; HIV-1; Humans; Immune activation; Low-level viremia (LLV); Lymphocyte Activation; Male; Pediatric HIV/AIDS; T-Lymphocyte Subsets - immunology; T-Lymphocytes - immunology; T-Lymphocytes, Regulatory - immunology; Thymic output; Viral Load; Viremia - drug therapy; Viremia - immunology; Viremia - virology; Young Adult; Low-level viremia (LLV); Pediatric HIV/AIDS; Immune activation; Thymic output; Antiretroviral therapy
• ISSN: 1201-9712; 1878-3511
• DOI: 10.1016/j.ijid.2019.12.001

•Low-level viremia (LLV) and virological failure (VF) led to immune activation.•LLV and VF patients showed comparable levels of thymic output and Treg subsets to virological suppression (VS) patients.•LLV did not impair the conversion of naïve to memory T cells among school-age children. Given the improvements in antiretroviral therapy (ART) in recent years, more pediatric HIV patients receiving ART are reaching adolescence and adulthood. This study investigated the influence of poor virological response (low-level viremia (LLV) and virological failure (VF)) on the immune system of these patients. HIV-infected, ART-experienced pediatric patients (n=206) were enrolled in this cross-sectional study. The patients were subdivided into school-age children/early adolescents, middle adolescents, and late adolescents/young adults according to their age, and further classified into virological suppression (VS), LLV, and VF groups according to plasma viral load (pVL) measurement. Thymic output, T cells subsets, and immune activation were analyzed by flow cytometry. Compared with VS patients, VF patients displayed decreased CD4+ T cell counts, while LLV and VS patients had comparable CD4+ T cell counts regardless of age. Compared with VS patients, LLV and VF patients had higher percentages of CD8+HLA-DR+ and CD8+CD38high T cells, and the immune activation was positively correlated with pVL in VF and LLV patients. Thymic output levels (CD31+) and regulatory T cell subpopulations in LLV and VF patients were comparable to those in VS patients. LLV patients showed comparable percentages of T cell subsets (TN, TCM, TEMRA, and TEM) as VS patients in all age groups. LLV causes excessive immune activation although it does not impair T cell recovery or naïve-to-memory T cell conversion in pediatric patients living with HIV. Therefore, T cell immune activation should be monitored at the management of LLV during ART.