A functional extracellular matrix biomaterial derived from ovine forestomach

• Published in: Biomaterials Vol. 31; no. 16; pp. 4517 - 4529
• Main Authors: Lun, Stan, Irvine, Sharleen M, Johnson, Keryn D, Fisher, Neil J, Floden, Evan W, Negron, Leonardo, Dempsey, Sandi G, McLaughlin, Rene J, Vasudevamurthy, Madhusudan, Ward, Brian R, May, Barnaby C.H
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 01.06.2010
• Subjects: Advanced Basic Science; Animals; Biocompatible Materials - chemistry; Biocompatible Materials - metabolism; Cell Adhesion; Cell culture; Cell Differentiation; Collagen; Collagen - metabolism; Dentistry; ECM (extracellular matrix); Extracellular Matrix - chemistry; Extracellular Matrix - metabolism; Fibroblast growth factor; Fibroblast Growth Factor 2 - metabolism; Humans; Laminin - metabolism; PC12 Cells; Protein Isoforms - metabolism; Rats; Regeneration - physiology; Sheep; Stomach - anatomy & histology; Stomach - chemistry; Tissue regeneration; Wound healing; ECM (extracellular matrix); Fibroblast growth factor; Cell culture; Wound healing; Tissue regeneration; Collagen
• ISSN: 0142-9612; 1878-5905
• DOI: 10.1016/j.biomaterials.2010.02.025

Abstract Extracellular matrix (ECM) based biomaterials have an established place as medical devices for wound healing and tissue regeneration. In the search for biomaterials we have identified ovine forestomach matrix (OFM), a thick, large format ECM which is biochemically diverse and biologically functional. OFM was purified using an osmotic process that was shown to reduce the cellularity of the ECM and aid tissue delamination. OFM produced using this technique was shown to retain residual basement membrane components, as evidence by the presence of laminin and collagen IV. The collagenous microarchitecture of OFM retained many components of native ECM including fibronectin, glycosaminoglycans, elastin and fibroblast growth factor basic. OFM was non-toxic to mammalian cells and supported fibroblast and keratinocyte migration, differentiation and infiltration. OFM is a culturally acceptable alternative to current collagen-based biomaterials and has immediate clinical applications in wound healing and tissue regeneration.