Proteomic analysis of secreted proteins from aseptically grown rice
To investigate proteins secreted from rice roots, rice plants were grown aseptically in an hydroculture system. Nano-LC–MS/MS analysis indicated that a large number of identified proteins belong to defense related proteins. [Display omitted] ► Secreted proteins were collected from root bathing solut...
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Published in | Phytochemistry (Oxford) Vol. 72; no. 4-5; pp. 312 - 320 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Amsterdam
Elsevier Ltd
01.04.2011
Elsevier |
Subjects | |
Online Access | Get full text |
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Summary: | To investigate proteins secreted from rice roots, rice plants were grown aseptically in an hydroculture system. Nano-LC–MS/MS analysis indicated that a large number of identified proteins belong to defense related proteins. [Display omitted]
► Secreted proteins were collected from root bathing solution of rice. ► Nano-LC–MS/MS succeeded to identify over 100 proteins. ► PR-proteins are constitutively secreted under aseptic conditions.
Plants are known to secrete a variety of compounds into the rhizosphere. These compounds are thought to play important roles in the regulation of soil chemical properties and soil microorganisms. To determine the composition of proteins secreted from rice roots, aseptic hydro culture was performed, and the collected proteins were analyzed. Over 100 proteins were identified; most were identified using the rice database (RAP-DB), and about 60% of the identified proteins were suspected to have a signal peptide. Functional categorization suggested that most were secondary metabolism- and defense-related proteins. Pathogenesis- and stress-related proteins were the major proteins found in the bathing solution under aseptic conditions. Thus, we propose that rice plants constitutively secrete a large variety of proteins to protect their roots against abiotic and/or biotic stresses in the environment. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0031-9422 1873-3700 |
DOI: | 10.1016/j.phytochem.2010.12.006 |