Cellulosic ethanol production on temperature-shift simultaneous saccharification and fermentation using the thermostable yeast Kluyveromyces marxianus CHY1612

• Published in: Bioprocess and biosystems engineering Vol. 35; no. 1-2; pp. 115 - 122
• Main Authors: Kang, Hyun-Woo, Kim, Yule, Kim, Seung-Wook, Choi, Gi-Wook
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 01.01.2012; Springer; Springer Nature B.V
• Subjects: Biofuels; Biofuels - microbiology; Biological and medical sciences; Biomass; Bioreactors - microbiology; Biotechnology; Cell Culture Techniques - methods; Cellulose; Cellulose - metabolism; Chemistry; Chemistry and Materials Science; Environmental Engineering/Biotechnology; Ethanol; Ethanol - isolation & purification; Ethanol - metabolism; Fermentation; Food Science; Fundamental and applied biological sciences. Psychology; Glucose - metabolism; Industrial and Production Engineering; Industrial Chemistry/Chemical Engineering; Kluyveromyces - classification; Kluyveromyces - metabolism; Methods. Procedures. Technologies; Microbial engineering. Fermentation and microbial culture technology; Original Paper; Temperature; Temperature effects; Yeast; Yeasts; Thermostable yeast; Temperature-shift; Barley straw; Bioethanol; Simultaneous saccharification and fermentation; Ascomycota; Temperature; Yeast; Ethanol; Cellulose; Saccharification; Fermentation; Thermal stability; Straw; Fungi; Production; Kluyveromyces marxianus
• ISSN: 1615-7591; 1615-7605
• DOI: 10.1007/s00449-011-0621-0

In cellulosic ethanol production, use of simultaneous saccharification and fermentation (SSF) has been suggested as the favorable strategy to reduce process costs. Although SSF has many advantages, a significant discrepancy still exists between the appropriate temperature for saccharification (45–50 °C) and fermentation (30–35 °C). In the present study, the potential of temperature-shift as a tool for SSF optimization for bioethanol production from cellulosic biomass was examined. Cellulosic ethanol production of the temperature-shift SSF (TS-SSF) from 16 w/v% biomass increased from 22.2 g/L to 34.3 g/L following a temperature shift from 45 to 35 °C compared with the constant temperature of 45 °C. The glucose conversion yield and ethanol production yield in the TS-SSF were 89.3% and 90.6%, respectively. At higher biomass loading (18 w/v%), ethanol production increased to 40.2 g/L with temperature-shift time within 24 h. These results demonstrated that the temperature-shift process enhances the saccharification ratio and the ethanol production yield in SSF, and the temperature-shift time for TS-SSF process can be changed according to the fermentation condition within 24 h.