A Smooth Muscle-Like Origin for Beige Adipocytes
Thermogenic UCP1-positive cells, which include brown and beige adipocytes, transform chemical energy into heat and increase whole-body energy expenditure. Using a ribosomal profiling approach, we present a comprehensive molecular description of brown and beige gene expression from multiple fat depot...
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Published in | Cell metabolism Vol. 19; no. 5; pp. 810 - 820 |
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Main Authors | , , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
Elsevier Inc
06.05.2014
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Subjects | |
Online Access | Get full text |
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Summary: | Thermogenic UCP1-positive cells, which include brown and beige adipocytes, transform chemical energy into heat and increase whole-body energy expenditure. Using a ribosomal profiling approach, we present a comprehensive molecular description of brown and beige gene expression from multiple fat depots in vivo. This UCP1-TRAP data set demonstrates striking similarities and important differences between these cell types, including a smooth muscle-like signature expressed by beige, but not classical brown, adipocytes. In vivo fate mapping using either a constitutive or an inducible Myh11-driven Cre demonstrates that at least a subset of beige cells arise from a smooth muscle-like origin. Finally, ectopic expression of PRDM16 converts bona fide vascular smooth muscle cells into Ucp1-positive adipocytes in vitro. These results establish a portrait of brown and beige adipocyte gene expression in vivo and identify a smooth muscle-like origin for beige cells.
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•Ribosomal profiling provides a molecular portrait of thermogenic adipocytes in vivo•Beige, but not classical brown cells, express smooth muscle-like genes•Smooth muscle-like cells are precursors for beige adipocytes in vivo•PRDM16 converts smooth muscle cells into thermogenic adipocytes in vitro
Long et al. use ribosomal profiling (TRAP) to analyze UCP1-positive cells and map similarities and differences between brown and beige adipocytes, including a “smooth muscle-like” signature for beige but not brown adipocytes. In vivo fate mapping reveal a smooth muscle-like origin for beige cells. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1550-4131 1932-7420 |
DOI: | 10.1016/j.cmet.2014.03.025 |