Continuous production of β-cyclodextrin from starch by highly stable cyclodextrin glycosyltransferase immobilized on chitosan

• Published in: Carbohydrate polymers Vol. 98; no. 2; pp. 1311 - 1316
• Main Authors: Schöffer, Jéssie da Natividade, Klein, Manuela Poletto, Rodrigues, Rafael C., Hertz, Plinho Francisco
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 06.11.2013; Elsevier
• Subjects: Applied sciences; Bacterial Proteins - chemistry; beta-cyclodextrin; beta-Cyclodextrins - chemical synthesis; Biocatalysis; biocatalysts; Biological and medical sciences; Biotechnology; Chitosan; Chitosan - chemistry; Cyclodextrin glycosyltransferase; cyclomaltodextrin glucanotransferase; Enzyme immobilization; Enzymes, Immobilized - chemistry; Exact sciences and technology; Fundamental and applied biological sciences. Psychology; Glucosyltransferases - chemistry; Glutaral - chemistry; Hydrogen-Ion Concentration; Immobilization of enzymes and other molecules; Immobilization techniques; Kinetics; Methods. Procedures. Technologies; Natural polymers; Packed bed reactor; Physicochemistry of polymers; starch; Starch - chemistry; Starch and polysaccharides; Temperature; Thermoanaerobacter; Thermoanaerobacter - chemistry; Thermoanaerobacter - enzymology; Cyclodextrin glycosyltransferase; Packed bed reactor; Enzyme immobilization; Chitosan; Enzymatic synthesis; Packed bed; Support; Enzyme; Starch; Transferases; Glycosyltransferases; Temperature effect; Immobilization; Experimental study; Continuous process; Cyclodextrin; Enzymatic activity; Covalent bond; pH; Oside polymer; Immobilized enzyme
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2013.07.044

•Cyclodextrin glycosyltransferase was covalently immobilized on chitosan spheres.•The derivative optimum temperature was 85°C and pH 5.0.•The immobilized biocatalyst retained 61% of activity after 100 cycles of batch.•High operational stability: 100h of continuous use, with maximum activity. Cyclodextrin glycosyltransferase (CGTase) from Thermoanaerobacter sp. was covalently immobilized on glutaraldehyde-activated chitosan spheres and used in a packed bed reactor to investigate the continuous production of β-cyclodextrin (β-CD). The optimum temperatures were 75°C and 85°C at pH 6.0, respectively for free and immobilized CGTase, and the optimum pH (5.0) was the same for both at 60°C. In the reactor, the effects of flow rate and substrate concentration in the β-CD production were evaluated. The optimum substrate concentration was 4% (w/v), maximizing the β-CD production (1.32g/L) in a flow rate of 3mL/min. In addition, the biocatalyst had good operational stability at 60°C, maintaining 61% of its initial activity after 100 cycles of batch and 100% after 100h of continuous use. These results suggest the possibility of using this immobilized biocatalyst in continuous production of CDs.