Thresholds for indirect DNA damage across cellular barriers for orthopaedic biomaterials

• Published in: Biomaterials Vol. 31; no. 16; pp. 4477 - 4483
• Main Authors: Parry, Michael C, Bhabra, Gevdeep, Sood, Aman, Machado, Filipa, Cartwright, Laura, Saunders, Margaret, Ingham, E, Newson, R, Blom, Ashley W, Case, Charles P
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 01.06.2010
• Subjects: Advanced Basic Science; Alloys - chemistry; Alloys - pharmacology; Animals; Arthroplasty; Arthroplasty, Replacement, Hip; Biocompatible Materials - pharmacology; Cell signalling; Cells, Cultured; Ceramics - chemistry; Ceramics - pharmacology; Chromium - chemistry; Chromium - pharmacology; Chromosome Aberrations - chemically induced; Cobalt - chemistry; Cobalt - pharmacology; Cobalt alloy; Dentistry; DNA - drug effects; DNA Damage; Female; Fibroblasts - cytology; Fibroblasts - drug effects; Fibroblasts - physiology; Genotoxicity; Humans; Karyotyping; Materials Testing; Metal Nanoparticles - chemistry; Nanoparticle; Orthopedics; Placenta - cytology; Placenta - drug effects; Placenta - metabolism; Pregnancy; Reproductive toxicity; Cell signalling; Arthroplasty; Nanoparticle; Reproductive toxicity; Cobalt alloy; Genotoxicity

Abstract Cobalt–chromium particles and ions can induce indirect DNA damage and chromosome aberrations in human cells on the other side of a cellular barrier in tissue culture. This occurs by intercellular signalling across the barrier. We now show that the threshold for this effect depends on the me...