Genome-wide identification and in-silico expression analysis of carotenoid cleavage oxygenases gene family in Oryza sativa (rice) in response to abiotic stress

• Published in: Frontiers in plant science Vol. 14; p. 1269995
• Main Authors: Haider, Muhammad Zeshan, Sami, Adnan, Shafiq, Muhammad, Anwar, Waheed, Ali, Sajid, Ali, Qurban, Muhammad, Sher, Manzoor, Irfan, Shahid, Muhammad Adnan, Ali, Daoud, Alarifi, Saud
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 25.10.2023
• Subjects: 9-cisepoxycarotenoid dioxygenases; apocarotenoids; carotenoid cleavage oxygenases; O. sativa; Oryza sativa; Plant Science; RNA seq analysis; Oryza sativa; carotenoid cleavage oxygenases; 9-cisepoxycarotenoid dioxygenases; transcriptome; O. sativa; abscisic acid; apocarotenoids; RNA seq analysis
• ISSN: 1664-462X; 1664-462X
• DOI: 10.3389/fpls.2023.1269995

Rice constitutes a foundational cereal and plays a vital role in the culinary sector. However, the detriments of abiotic stress on rice quality and productivity are noteworthy. Carotenoid cleavage oxygenases ( CCO ) hold vital importance as they enable the particular breakdown of carotenoids and significantly contribute towards the growth and response to abiotic stress in rice. Due to the insufficient information regarding rice CCOs and their potential role in abiotic stress, their utilization in stress-resistant genetic breeding remains limited. The current research identified 16 CCO genes within the Oryza sativa japonica group. These Os CCO genes can be bifurcated into three categories based on their conserved sequences: NCEDs (9-Cis-epoxycarotenoid dioxygenases), CCDs (Carotenoid cleavage dioxygenases) and CCD-like (Carotenoid cleavage dioxygenases-like). Conserved motifs were found in the OsCCO gene sequence via MEME analysis and multiple sequence alignment. Stress-related cis-elements were detected in the promoter regions of OsCCOs genes, indicating their involvement in stress response. Additionally, the promoters of these genes had various components related to plant light, development, and hormone responsiveness, suggesting they may be responsive to plant hormones and involved in developmental processes. MicroRNAs play a pivotal role in the regulation of these 16 genes, underscoring their significance in rice gene regulation. Transcriptome data analysis suggests a tissue-specific expression pattern for rice CCOs . Only OsNCED6 and OsNCED10 significantly up-regulated during salt stress, as per RNA seq analyses. CCD7 and CCD8 levels were also higher in the CCD group during the inflorescence growth stage. This provides insight into the function of rice CCOs in abiotic stress response and identifies possible genes that could be beneficial for stress-resistant breeding.