Highly Sensitive and Robust Capillary Electrophoresis-Electrospray Ionization-Mass Spectrometry: Interfaces, Preconcentration Techniques and Applications

Capillary electrophoresis (CE) coupled with mass spectrometry (MS) through electrospray ionization (ESI) is a promising alternative technique to liquid chromatography-ESI-MS (LC-ESI-MS) due to its high separation efficiency and high detection sensitivity. A sensitive and robust interface is essentia...

Full description

Saved in:
Bibliographic Details
Published inReviews in analytical chemistry Vol. 39; no. 1; pp. 45 - 55
Main Authors Wu, Huanming, Tang, Keqi
Format Journal Article
LanguageEnglish
Published Berlin De Gruyter 01.01.2020
Walter de Gruyter GmbH
Subjects
Capillary electrophoresis
CE-ESI-MS
Electrophoresis
Electrospraying
interface
Ionization
Ions
Liquid chromatography
Mass spectrometry
Peptides
Proteins
robust
Robustness
sample preconcentration technique
Scientific imaging
sensitive
Sensitivity
Separation
Spectroscopy
Online AccessGet full text

Cover

More Information
Summary:Capillary electrophoresis (CE) coupled with mass spectrometry (MS) through electrospray ionization (ESI) is a promising alternative technique to liquid chromatography-ESI-MS (LC-ESI-MS) due to its high separation efficiency and high detection sensitivity. A sensitive and robust interface is essential in CE-ESI-MS. Continued development of CE-ESI-MS interfaces in the last decade, including junction-at-the-tip interfaces and sheathless interfaces, has improved the sensitivity and robustness of CE-ESI-MS significantly. The limited loading capacity of CE, one of major reasons that limits the utility of CE as a routine separation method, has also been addressed effectively by the use of in-capillary sample preconcentration techniques, such as transient CITP/CZE and dynamic pH junction. CE-ESI-MS could yield higher sensitivity as compared with the conventional LC-ESI-MS, and, therefore, is capable of identifying more proteins and peptides when the sample amount is very limited, such as single cell analysis. To improve the protein sequence coverage, CE-ESI-MS can also be used as a complementary technique to LC-ESI-MS, or combined with reversed phase LC to form a two dimensional separation technique. CE-ESI-MS is also effective in quantifying targeted peptides/proteins in complex bio-matrix.
ISSN:0793-0135
2191-0189
DOI:10.1515/revac-2020-0112