Dynamics of Wolbachia Populations in Transfected Lines of Trichogramma

• Published in: Journal of invertebrate pathology Vol. 76; no. 1; pp. 20 - 25
• Main Authors: Pintureau, Bernard, Grenier, Simon, Boléat, Bénédicte, Lassablière, François, Heddi, Abdelaziz, Khatchadourian, Chaqué
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Inc 01.07.2000; Elsevier
• Subjects: Animal, plant and microbial ecology; Animals; Bacteria Rickettsiaceae; Biological and medical sciences; DNA detection; egg parasitoid; endosymbiont; Female; Fundamental and applied biological sciences. Psychology; Genetic Variation; horizontal transfer; Hymenoptera Trichogrammatidae; Microbial ecology; Microinjections; Normal microflora of man and animals. Rumen; Ovary - microbiology; Solutions; Symbiosis; thelytoky; Wasps - growth & development; Wasps - microbiology; Wolbachia - genetics; Wolbachia - growth & development; Wolbachia - isolation & purification; Bacteria Rickettsiaceae; egg parasitoid; horizontal transfer; DNA detection; Hymenoptera Trichogrammatidae; thelytoky; endosymbiont; Animal microorganism relation; Genetic variability; Insecta; Transmission from animal to animal; Chalcidoidea; Trichogrammatidae; Trichogramma; Bacteria; Developmental stage; Localization; Adaptation; Wolbachieae; Symbionte; Symbiotic relation; Egg; Host; Rickettsiales; Cell line; Microinjection; Parasitoid; Wolbachia; Arthropoda; Population dynamics; Hymenoptera; Invertebrata; Population density
• ISSN: 0022-2011; 1096-0805
• DOI: 10.1006/jipa.2000.4953

Fluorescence in situ hybridization was tested to specifically detect symbionts of the genus Wolbachia in Trichogramma and to allow for semiquantitative estimations of symbiont abundance. Extraction solutions used for horizontal transfers of symbionts contain a high abundance of Wolbachia, but Wolbachia have a low and decreasing abundance in microinjected lines (transfected lines). Moreover, eggs of microinjected lines were shown to be polymorphic for the infection. In naturally infected lines, Wolbachia are localized at the posterior pole of the eggs; they are scattered during the early stages of larval development and then concentrated in the ovaries at the end of the female pupal development. Scattering and concentration are probably not active but rather the result of replications or morphogenesis. Conversely, Wolbachia are not concentrated at the posterior pole of eggs in microinjected lines. Comparison of the within-family and between-family variances of the symbiont abundance in a microinjected line did not lead us to conclude that this character shows a genetic variability.