A sensitive and specific nanosensor for monitoring extracellular potassium levels in the brain

• Published in: Nature nanotechnology Vol. 15; no. 4; pp. 321 - 330
• Main Authors: Liu, Jianan, Li, Fangyuan, Wang, Yi, Pan, Limin, Lin, Peihua, Zhang, Bo, Zheng, Yanrong, Xu, Yingwei, Liao, Hongwei, Ko, Giho, Fei, Fan, Xu, Cenglin, Du, Yang, Shin, Kwangsoo, Kim, Dokyoon, Jang, Sung-Soo, Chung, Hee Jung, Tian, He, Wang, Qi, Guo, Wei, Nam, Jwa-Min, Chen, Zhong, Hyeon, Taeghwan, Ling, Daishun
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.04.2020; Nature Publishing Group
• Subjects: 140/146; 631/61/350; 639/301/357; 639/925; Alzheimer Disease - metabolism; Alzheimer Disease - pathology; Alzheimer's disease; Animals; Brain; Cations; Change detection; Chemistry and Materials Science; Convulsions & seizures; Diffusion layers; Epilepsy; HEK293 Cells; Hippocampus - metabolism; Hippocampus - pathology; Humans; Ions; Male; Mapping; Materials Science; Membrane potential; Membrane Potentials; Membranes; Monitoring; Nanoparticles; Nanosensors; Nanotechnology; Nanotechnology and Microengineering; Neurodegenerative diseases; Neurological diseases; Potassium; Potassium - metabolism; Rats; Rats, Sprague-Dawley; Seizures; Seizures - metabolism; Seizures - pathology; Silica; Silicon dioxide
• ISSN: 1748-3387; 1748-3395
• DOI: 10.1038/s41565-020-0634-4

Extracellular potassium concentration affects the membrane potential of neurons, and, thus, neuronal activity. Indeed, alterations of potassium levels can be related to neurological disorders, such as epilepsy and Alzheimer’s disease, and, therefore, selectively detecting extracellular potassium would allow the monitoring of disease. However, currently available optical reporters are not capable of detecting small changes in potassium, in particular, in freely moving animals. Furthermore, they are susceptible to interference from sodium ions. Here, we report a highly sensitive and specific potassium nanosensor that can monitor potassium changes in the brain of freely moving mice undergoing epileptic seizures. An optical potassium indicator is embedded in mesoporous silica nanoparticles, which are shielded by an ultrathin layer of a potassium-permeable membrane, which prevents diffusion of other cations and allows the specific capturing of potassium ions. The shielded nanosensor enables the spatial mapping of potassium ion release in the hippocampus of freely moving mice. Extracellular potassium levels in the brain can be correlated to neural activity. A selective potassium sensor, in which cations other than potassium are shielded by a membrane, can measure potassium concentration changes in the brain of freely moving mice undergoing epileptic seizures.