Petroleum and polycyclic aromatic hydrocarbons (PAHs) degradation and naphthalene metabolism in Streptomyces sp. (ERI-CPDA-1) isolated from oil contaminated soil

• Published in: Bioresource technology Vol. 112; pp. 83 - 90
• Main Authors: Balachandran, C., Duraipandiyan, V., Balakrishna, K., Ignacimuthu, S.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.05.2012
• Subjects: Biodegradation; Biodegradation, Environmental - drug effects; Biomass; Carbon - pharmacology; GC–MS; Metabolic Networks and Pathways - drug effects; Metabolic pathway; Molecular Sequence Data; Naphthalenes - metabolism; Oils - chemistry; Petroleum - microbiology; Phenanthrenes - metabolism; Phylogeny; Polycyclic aromatic hydrocarbons; Polycyclic Aromatic Hydrocarbons - metabolism; Soil Microbiology; Soil Pollutants - analysis; Streptomyces; Streptomyces - drug effects; Streptomyces - growth & development; Streptomyces - isolation & purification; Streptomyces - metabolism; Streptomyces sp; Substrate Specificity - drug effects; India; Biodegradation; GC–MS; Metabolic pathway; Polycyclic aromatic hydrocarbons; Streptomyces sp
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2012.02.059

► Polycyclic aromatic hydrocarbons degrading Streptomyces sp. ERI-CPDA-1 was isolated. ► The degradation efficiencies ranged from 98.25% for diesel oil in 7days at 30°C. ► 99.14% for naphthalene and 17.5% for phenanthrene in 7days at 30°C. ► Metabolic pathway was also worked out for naphthalene degradation. ► This isolate can be exploited for the development of biodegradation agent. Petroleum and polycyclic aromatic hydrocarbons (PAHs) degrading Streptomyces sp. isolate ERI-CPDA-1 was recovered from oil contaminated soil in Chennai, India. The degradation efficiencies were examined by GC-FID and the results showed that the isolate could remove 98.25% diesel oil, 99.14% naphthalene and 17.5% phenanthrene in 7days at 30°C (0.1%). ERI-CPDA-1 was able to degrade naphthalene, phenanthrene and diesel oil and grow on petrol, diesel, kerosene, benzene, pyridine, methanol, ethanol, cyclohexane, tween-80, xylene, DMSO and toluene using them as sole carbon source. Effects of environmental condition on degradation of hydrocarbons (diesel oil, naphthalene and phenanthrene) were also studied at different pH, NaCl, temperature, concentration and incubation time. Degradation pathway for naphthalene has been proposed. Degradation metabolites were identified using GC–MS analysis of ethyl acetate extract of the cell free culture. The degradation products detected were benzaldehyde, catechol, phenylacetic acid and protocatechuic acid.