Apolipoprotein A-I isoforms in human lymph: effect of fat absorption

• Published in: Journal of lipid research Vol. 24; no. 6; pp. 731 - 736
• Main Authors: Ghiselli, G, Schaefer, E.J, Light, J.A, Brewer, H.B. Jr
• Format: Journal Article
• Language: English
• Published: United States Elsevier 01.06.1983
• Subjects: Apolipoprotein A-I; Apolipoproteins - isolation & purification; Apolipoproteins - metabolism; Cholesterol - isolation & purification; Dietary Fats - metabolism; Electrophoresis, Polyacrylamide Gel; human nutrition; Humans; Immunodiffusion; Intestinal Absorption; Lipoproteins, HDL - isolation & purification; Lymph - physiology; nutrition physiology; Structure-Activity Relationship; Triglycerides - isolation & purification
• ISSN: 0022-2275; 1539-7262
• DOI: 10.1016/S0022-2275(20)37946-3

The effect of fat feeding (100 g of cream) on the apoA-I isoproteins distribution has been analyzed by two-dimensional gel electrophoresis in the chylomicrons, VLDL, LDL, and HDL isolated from the thoracic duct lymph of patients undergoing lymph drainage for immunosuppression, Isoforms apoA-I3 and apoA-I4 are the most abundant apoA-I isoproteins in plasma lipoproteins as well as in lymph lipoproteins collected in the fasting state. Fat feeding, on the other hand, results in a marked change in the apoA-I isoform pattern in lymph chylomicrons and VLDL, with a significant increase in the relative concentration of the apoA-I1 isoform. As a result the total concentration of this isoprotein in the lymph increased. The data indicate that fat feeding is associated with major changes in the distribution of the apoA-I isoforms in the lymph (d less than 1.006 g/ml lipoproteins), which may be of significance in their plasma catabolism.