Shared gut, but distinct oral microbiota composition in primary Sjögren's syndrome and systemic lupus erythematosus

• Published in: Journal of autoimmunity Vol. 97; pp. 77 - 87
• Main Authors: van der Meulen, Taco A., Harmsen, Hermie J.M., Vila, Arnau Vich, Kurilshikov, Alexander, Liefers, Silvia C., Zhernakova, Alexandra, Fu, Jingyuan, Wijmenga, Cisca, Weersma, Rinse K., de Leeuw, Karina, Bootsma, Hendrika, Spijkervet, Fred K.L., Vissink, Arjan, Kroese, Frans G.M.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.02.2019
• Subjects: Adult; Biodiversity; Case-Control Studies; Disease Susceptibility; Dysbiosis; Feces - microbiology; Female; Gastrointestinal Microbiome; Humans; Lupus Erythematosus, Systemic - diagnosis; Lupus Erythematosus, Systemic - etiology; Lupus Erythematosus, Systemic - metabolism; Male; Metagenome; Metagenomics - methods; Microbiota; Middle Aged; Mouth Mucosa - microbiology; Phenotype; RNA, Ribosomal, 16S - genetics; Sjogren's Syndrome - diagnosis; Sjogren's Syndrome - etiology; Sjogren's Syndrome - metabolism
• ISSN: 0896-8411; 1095-9157
• DOI: 10.1016/j.jaut.2018.10.009

Alterations in the microbiota composition of the gastro-intestinal tract are suspected to be involved in the etiopathogenesis of two closely related systemic inflammatory autoimmune diseases: primary Sjögren's syndrome (pSS) and systemic lupus erythematosus (SLE). Our objective was to assess whether alterations in gut and oral microbiota compositions are specific for pSS and SLE. 16S ribosomal RNA gene sequencing was performed on fecal samples from 39 pSS patients, 30 SLE patients and 965 individuals from the general population, as well as on buccal swab and oral washing samples from the same pSS and SLE patients. Alpha-diversity, beta-diversity and relative abundance of individual bacteria were used as outcome measures. Multivariate analyses were performed to test associations between individual bacteria and disease phenotype, taking age, sex, body-mass index, proton-pump inhibitor use and sequencing-depth into account as possible confounding factors. Fecal microbiota composition from pSS and SLE patients differed significantly from population controls, but not between pSS and SLE. pSS and SLE patients were characterized by lower bacterial richness, lower Firmicutes/Bacteroidetes ratio and higher relative abundance of Bacteroides species in fecal samples compared with population controls. Oral microbiota composition differed significantly between pSS patients and SLE patients, which could partially be explained by oral dryness in pSS patients. pSS and SLE patients share similar alterations in gut microbiota composition, distinguishing patients from individuals in the general population, while oral microbiota composition shows disease-specific differences between pSS and SLE patients. •pSS and SLE patients share similar alterations in the gut microbiome.•Low richness and high Bacteroides characterizes the pSS/SLE gut microbiome.•The oral microbiome of pSS and SLE patients shows disease-specific changes.•Ro60/SSA-positivity is not associated with bacteria in the gut or oral microbiome.