Low doses of diagnostic energy X-rays protect against neoplastic transformation in vitro

• Published in: International journal of radiation biology Vol. 79; no. 4; pp. 235 - 240
• Main Authors: REDPATH, J. L., LU, Q., LAO, X., MOLLOI, S., ELMORE, E.
• Format: Journal Article
• Language: English
• Published: London Informa UK Ltd 01.04.2003; Taylor & Francis
• Subjects: Biological and medical sciences; Cell Transformation, Neoplastic - radiation effects; Dose-Response Relationship, Radiation; Gamma Rays; HeLa Cells; Humans; Hybrid Cells - radiation effects; In Vitro Techniques; Medical sciences; Neoplasms, Radiation-Induced - etiology; Risk Factors; X-Rays
• ISSN: 0955-3002; 1362-3095
• DOI: 10.1080/0955300031000096306

Purpose : To investigate the effect of low doses of 60 kVp X-rays on in vitro transformation frequency. Materials and methods : HeLa ×skin fibroblast human hybrid cells were used to assay transformation from the non-tumorigenic to the tumorigenic phenotype. Subconfluent cultures of cells were exposed to a range of doses of 60 kVp X-rays and seeded for assay of transformation after 24 h post-irradiation holding. Experiments were repeated at least three times and the data pooled for analysis. Transformation frequencies were compared with those of sham-irradiated controls. Results : At doses < 1 cGy, the observed transformation frequencies were significantly less than those seen in unirradiated cells. Conclusion : Low doses (< 1 cGy) of 60 kVp X-rays protect HeLa ×skin fibroblast human hybrid cells against neoplastic transformation in vitro.