An integrated nitrogen utilization gene network and transcriptome analysis reveal candidate genes in response to nitrogen deficiency in Brassica napus
Nitrogen (N) is an essential factor for crop yield. Here, we characterized 605 genes from 25 gene families that form the complex gene networks of N utilization pathway in . We found unequal gene distribution between the A - and C -sub-genomes, and that genes derived from were more retained. Transcri...
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Published in | Frontiers in plant science Vol. 14; p. 1187552 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
Frontiers Media S.A
09.05.2023
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Subjects | |
Online Access | Get full text |
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Summary: | Nitrogen (N) is an essential factor for crop yield. Here, we characterized 605 genes from 25 gene families that form the complex gene networks of N utilization pathway in
. We found unequal gene distribution between the A
- and C
-sub-genomes, and that genes derived from
were more retained. Transcriptome analysis indicated that N utilization pathway gene activity shifted in a spatio-temporal manner in
. A low N (LN) stress RNA-seq of
seedling leaves and roots was generated, which proved that most N utilization related genes were sensitive to LN stress, thereby forming co-expression network modules. Nine candidate genes in N utilization pathway were confirmed to be significantly induced under N deficiency conditions in
roots, indicating their potential roles in LN stress response process. Analyses of 22 representative species confirmed that the N utilization gene networks were widely present in plants ranging from Chlorophyta to angiosperms with a rapid expansion trend. Consistent with
, the genes in this pathway commonly showed a wide and conserved expression profile in response to N stress in other plants. The network, genes, and gene-regulatory modules identified here represent resources that may enhance the N utilization efficiency or the LN tolerance of
. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Reviewed by: Dong-Liang Huang, Guangxi Academy of Agricultural Sciences, China; Aria Dolatabadian, University of Western Australia, Australia Edited by: Ting Xiang Tina Neik, University of Nottingham Malaysia Campus, Malaysia |
ISSN: | 1664-462X 1664-462X |
DOI: | 10.3389/fpls.2023.1187552 |