Determination of the Antiallergic Agent KB-2413 in Plasma by Means of Capillary Gas Chromatography with a Nitrogen-Sensitive Detector

A gas chromatographic method for the quantitative determination of the new antiallergic agent, 1-(2-ethoxyethyl)-2-(4-methyl-1-homopiperazinyl)benzimidazole difumarate (KB-2413), in plasma has been developed. This method, which was based on solvent extraction and flexible fusedsilica capillary gas c...

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Published inChemical & pharmaceutical bulletin Vol. 34; no. 3; pp. 1168 - 1171
Main Authors HAMADA, TSUKASA, KADOWAKI, MASANA, NAKAMURA, YURIKO, AWATA, NORIO
Format Journal Article
LanguageEnglish
Published Tokyo The Pharmaceutical Society of Japan 01.03.1986
Maruzen
Japan Science and Technology Agency
Subjects
Analysis
Animals
Benzimidazoles - blood
Biological and medical sciences
Chromatography, Gas
Dogs
General pharmacology
Histamine H1 Antagonists - blood
Male
Medical sciences
Pharmacology. Drug treatments
plasma
Capillary column
Assay
Gas chromatography
Biological fluid
Antiallergic
Blood plasma
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Summary:A gas chromatographic method for the quantitative determination of the new antiallergic agent, 1-(2-ethoxyethyl)-2-(4-methyl-1-homopiperazinyl)benzimidazole difumarate (KB-2413), in plasma has been developed. This method, which was based on solvent extraction and flexible fusedsilica capillary gas chromatography with a nitrogen-sensitive detector and a moving needle solvent cut sample injector, is simple, sensitive and selective.The detection limit was about 1 ng (as free base)/ml and the calibration curve was linear over the range of 2 to 100 ng/ml. Moreover, the intra- and inter-assay coefficients of variation for the determination of KB-2413 were 3.1-4.0% and 1.5-6.7%, respectively. This assay was applied to the determination of the plasma concentration of KB-2413 base after oral administration to dogs.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
ISSN:0009-2363
1347-5223
DOI:10.1248/cpb.34.1168