Structural contributions of blocked or grafted poly(2-dimethylaminoethyl methacrylate) on PEGylated polycaprolactone nanoparticles in siRNA delivery

• Published in: Biomaterials Vol. 32; no. 33; pp. 8730 - 8742
• Main Authors: Lin, Daoshu, Huang, Yuanyu, Jiang, Qian, Zhang, Wendi, Yue, Xinye, Guo, Shutao, Xiao, Ping, Du, Quan, Xing, Jinfeng, Deng, Liandong, Liang, Zicai, Dong, Anjie
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 01.11.2011
• Subjects: Advanced Basic Science; Animals; Base Sequence; Cationic polymer nanoparticles; Dentistry; Electrophoresis, Agar Gel; HeLa Cells; Humans; Male; Methacrylates - chemistry; Mice; Mice, Inbred C57BL; Molecular Structure; Nanoparticles; Non-viral carriers; Nylons - chemistry; PDMAEMA; PEG; Polyesters - chemistry; RNA, Small Interfering - administration & dosage; siRNA delivery; siRNA delivery; PEG; Cationic polymer nanoparticles; PDMAEMA; Non-viral carriers
• ISSN: 0142-9612; 1878-5905
• DOI: 10.1016/j.biomaterials.2011.07.089

Abstract The multiformity in polymer structure and conformation design provides a great potential in improving the gene silencing efficiency of siRNA by polymer vectors. In order to provide information on the polymer design for siRNA delivery, the structural contributions of blocked or grafted poly(2-dimethylaminoethyl methacrylate) on PEGylated polycaprolactone nanoparticles (NPs) in siRNA delivery were studied. Herein, two kinds of self-assembly nanoparticles (NPs) formed by amphiphilic cationic polymers, methoxy poly(ethylene glycol)-block-polycaprolactone-block-poly(2-dimethylaminoethyl methacrylate) (mPEG-PCL- b -PDMAEMA, PEC b D) and methoxy poly(ethylene glycol)-block-(polycaprolactone-graft-poly(2-dimethylaminoethyl methacrylate)) (mPEG-PCL- g -PDMAEMA, PEC g D), were used to deliver siRNA for in vitro and in vivo studies. The physiochemical properties including size and zeta potential of PEC b D NPs/siRNA and PEC g D NPs/siRNA complexes were characterized. In vitro cytotoxicity, cellular uptake and siRNA knockdown efficiency were evaluated in HeLa-Luc cells. The endosome escape and intracellular distribution of PEC b D NPs/siRNA and PEC g D NPs/siRNA in HeLa-Luc cells were also observed. In vivo polymer mediated siRNA delivery and the complexes distribution in isolated organs were studied using mice and tumor-bearing mice. At the same total degree of polymerization (DP) of DMAEMA, PEC g D NPs/siRNA complexes possessed higher zeta potentials than PEC b D NPs/siRNA complexes (at the same N/P ratio), which may be the reason that PEC g D NPs/siRNA complexes can deliver more siRNA into the cytoplasm and lead to higher in vitro luciferase and lamin A/C silencing efficiency than PEC b D NPs/siRNA complexes. The in vivo imaging measurement and histochemical analysis also confirmed that siRNA could be delivered to lungs, livers, pancreas and HeLa-Luc tumors more efficiently by PEC g D NPs than PEC b D NPs. Meanwhile, the PDMAEMA chains of PEC g D could be shortened which provides benefits for clearing. Therefore, PEC g D NPs have great potential to be used as efficient non-viral carriers for in vivo siRNA delivery.