Isolation and Identification Antagonistic Bacterium Paenibacillus tianmuensis YM002 against Acidovorax citrulli

In this study, we aimed to screen antagonistic microorganisms against , the causal agent of bacterial fruit blotch, which is known to induce sever diseases in cucurbit crops. From 240 bacterial strains isolated, only one unknown bacterial isolate, named YM002, showed significant antagonistic activit...

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Published inFrontiers in plant science Vol. 14; p. 1173695
Main Authors Koo, Young Mo, Heo, A Yeong, Choi, Hyong Woo
Format Journal Article
LanguageEnglish
Published Switzerland Frontiers Media S.A 12.06.2023
Subjects
biological control
cucumber
induced resistance
Paenibacillus tianmuensis
PGPR
Plant Science
Paenibacillus tianmuensis
biological control
induced resistance
PGPR
cucumber
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Summary:In this study, we aimed to screen antagonistic microorganisms against , the causal agent of bacterial fruit blotch, which is known to induce sever diseases in cucurbit crops. From 240 bacterial strains isolated, only one unknown bacterial isolate, named YM002, showed significant antagonistic activity against . KACC17909. Further experiments revealed that YM002 shows antagonistic activity against all tested . strains, including KACC17000, KACC17001 and KACC17005, to different degrees. The phylogenetic analysis of 16S rRNA sequences identified YM002 as . Importantly, pretreatment of cucumber ( ) leaves with YM002 enhanced disease resistance as observed by significantly reduced necrotic symptom development and bacterial growth. YM002-induced resistance accompanied by enhanced expression of defense-related genes, such as , and . Importantly, culture filtrate of YM002 significantly suppressed biofilm formation and swimming motility of , which is indispensable for its full virulence. In addition to its antagonistic activity, YM002 showed a various plant growth promotion (PGP)-related traits, such as production of ammonia production, amylase production, ACC deaminase production, inodole-3-acetic acid production, extracellular protease production, siderophore production, and zinc solubilization activities. Indeed, treatment of cucumber roots with YM002 significantly enhanced plant growth parameters, such as fresh and dry weight of leaves or roots. This study suggests the potential of YM002 as an effective PGPR with biological control activity against in cucumber plants.
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Edited by: Ágnes Szepesi, University of Szeged, Hungary
Reviewed by: Eduardo Valencia-Cantero, Michoacana University of San Nicolás de Hidalgo, Mexico; Youn-Sig Kwak, Gyeongsang National University, Republic of Korea; Christos Zamioudis, Democritus University of Thrace, Greece
ISSN:1664-462X
1664-462X
DOI:10.3389/fpls.2023.1173695