A Tumor-Associated Fibronectin Isoform Generated by Alternative Splicing of Messenger RNA Precursors

Fibronectin (FN) represents the mixture of a number of structurally different molecules (isoforms) whose make-up varies depending on the FN sources. FN from cultured transformed human cells has a very different isoform composition with respect to its normal counterpart. In fact, SV-40-transformed WI...

Full description

Saved in:
Bibliographic Details
Published inThe Journal of cell biology Vol. 108; no. 3; pp. 1139 - 1148
Main Authors Carnemolla, Barbara, Balza, Enrica, Siri, Annalisa, Zardi, Luciano, Nicotra, Maria Rita, Bigotti, Aldo, Natali, Pier Giorgio
Format Journal Article
LanguageEnglish
Published New York, NY Rockefeller University Press 01.03.1989
The Rockefeller University Press
Subjects
Online AccessGet full text

Cover

Loading…
Abstract Fibronectin (FN) represents the mixture of a number of structurally different molecules (isoforms) whose make-up varies depending on the FN sources. FN from cultured transformed human cells has a very different isoform composition with respect to its normal counterpart. In fact, SV-40-transformed WI-38VAI3 human fibroblasts produce high levels of a FN isoform (B-FN) which is very poorly expressed in their normal, WI-38, counterpart. We have recently demonstrated that the B-FN isoform derives from a differential splicing pattern of the FN primary transcript which leads, in transformed cells, to a high level expression of the exon ED-B. Here we report on the production and characterization of a monoclonal antibody (BC-1) which recognizes an epitope within the protein sequence coded for by the ED-B exon. This monoclonal antibody makes it possible to carry out immunohistochemical analysis of the distribution of the ED-B-containing FN isoform (B-FN) in human tissues. The results show that while in normal, adult, human tissues total FN has a widespread distribution, the B-FN isoform is restricted only to synovial cells, to some vessels and areas of the interstitium of the ovary, and to the myometrium. On the contrary, the B-FN isoform has a much greater expression in fetal and tumor tissues. These results demonstrate that, in vivo, different FN isoforms have a differential distribution and indicate that the B-FN isoform may play a role in ontogenesis and oncogenetic processes.
AbstractList Fibronectin (FN) represents the mixture of a number of structurally different molecules (isoforms) whose make-up varies depending on the FN sources. FN from cultured transformed human cells has a very different isoform composition with respect to its normal counterpart. In fact, SV-40-transformed WI-38VAI3 human fibroblasts produce high levels of a FN isoform (B-FN) which is very poorly expressed in their normal, WI-38, counterpart. We have recently demonstrated that the B-FN isoform derives from a differential splicing pattern of the FN primary transcript which leads, in transformed cells, to a high level expression of the exon ED-B. Here we report on the production and characterization of a monoclonal antibody (BC-1) which recognizes an epitope within the protein sequence coded for by the ED-B exon. This monoclonal antibody makes it possible to carry out immunohistochemical analysis of the distribution of the ED-B-containing FN isoform (B-FN) in human tissues. The results show that while in normal, adult, human tissues total FN has a widespread distribution, the B-FN isoform is restricted only to synovial cells, to some vessels and areas of the interstitium of the ovary, and to the myometrium. On the contrary, the B-FN isoform has a much greater expression in fetal and tumor tissues. These results demonstrate that, in vivo, different FN isoforms have a differential distribution and indicate that the B-FN isoform may play a role in ontogenesis and oncogenetic processes.
Fibronectin (FN) represents the mixture of a number of structurally different molecules (isoforms) whose make-up varies depending on the FN sources. FN from cultured transformed human cells has a very different isoform composition with respect to its normal counterpart. In fact, SV-40-transformed WI-38VAI3 human fibroblasts produce high levels of a FN isoform (B-FN) which is very poorly expressed in their normal, WI-38, counterpart. We have recently demonstrated that the B-FN isoform derives from a differential splicing pattern of the FN primary transcript which leads, in transformed cells, to a high level expression of the exon ED-B (Zardi, L., B. Carnemolla, A. Siri, T. E. Petersen, G. Paolella, G. Sebastio, and F. E. Baralle. 1987. EMBO (Eur. Mol. Biol. Organ.) J. 6:2337-2342). Here we report on the production and characterization of a monoclonal antibody (BC-1) which recognizes an epitope within the protein sequence coded for by the ED-B exon. This monoclonal antibody makes it possible to carry out immunohistochemical analysis of the distribution of the ED-B-containing FN isoform (B-FN) in human tissues. The results show that while in normal, adult, human tissues total FN has a widespread distribution, the B-FN isoform is restricted only to synovial cells, to some vessels and areas of the interstitium of the ovary, and to the myometrium. On the contrary, the B-FN isoform has a much greater expression in fetal and tumor tissues. These results demonstrate that, in vivo, different FN isoforms have a differential distribution and indicate that the B-FN isoform may play a role in ontogenesis and oncogenetic processes.
The authors have recently demonstrated that the B-FN isoform derives from a differential splicing pattern of the FN primary transcript which leads, in transformed cells, to a high level expression of the exon ED-B. Here the authors report on the production and characterization of a monoclonal antibody (BC-1) which recognizes an epitope within the protein sequence coded for by the ED-B exon. This monoclonal antibody makes it possible to carry out immunohistochemical analysis of the distribution of the ED-B-containing FN isoform (B-FN) in human tissues. The results show that while in normal, adult, human tissues total FN has a widespread distribution, the B-FN isoform is restricted only to synovial cells, to some vessels and areas of the interstitium of the ovary, and to the myometrium.
Author Zardi, Luciano
Nicotra, Maria Rita
Bigotti, Aldo
Carnemolla, Barbara
Siri, Annalisa
Balza, Enrica
Natali, Pier Giorgio
Author_xml – sequence: 1
  givenname: Barbara
  surname: Carnemolla
  fullname: Carnemolla, Barbara
– sequence: 2
  givenname: Enrica
  surname: Balza
  fullname: Balza, Enrica
– sequence: 3
  givenname: Annalisa
  surname: Siri
  fullname: Siri, Annalisa
– sequence: 4
  givenname: Luciano
  surname: Zardi
  fullname: Zardi, Luciano
– sequence: 5
  givenname: Maria Rita
  surname: Nicotra
  fullname: Nicotra, Maria Rita
– sequence: 6
  givenname: Aldo
  surname: Bigotti
  fullname: Bigotti, Aldo
– sequence: 7
  givenname: Pier Giorgio
  surname: Natali
  fullname: Natali, Pier Giorgio
BackLink http://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7196557$$DView record in Pascal Francis
https://www.ncbi.nlm.nih.gov/pubmed/2646306$$D View this record in MEDLINE/PubMed
BookMark eNqFkUtr3DAUhUVJSSdJt121oEXpzlPJetjeFExoHpA-yGMtJPl6qsGWJpIdyL-vJjNM21VXunA-nfs4J-jIBw8IvaNkSUnNPq-t2RZLtqSUNa_QggpOippycoQWhJS0aEQp3qCTlNaEEF5xdoyOS8klI3KBuhbfz2OIRZtSsE5P0OELZ2JuYifn8XUKfYgjvgQP8UU1z7gdJoheT-4J8N1mcNb5FQ49_gYpgV9BxLffW_wzgp1jCjGdode9HhK83b-n6OHi6_35VXHz4_L6vL0pLJdyKiSnpTE6z8sN7YngjWAcGCG0rxgXXQ-2lNCZUtRCiKojhvEepNYVsUaakp2iLzvfzWxG6Cz4KepBbaIbdXxWQTv1r-LdL7UKT6qkVLCGZoNPe4MYHmdIkxpdsjAM2kOYk6rqWvJ8xf-C2U7KkrEMLnegjSGlCP1hGkrUNkCVA9wWiqltgPnDh793OOD7xLL-ca_rZPXQR-2tSwesoo3Mt8nY-x22TlOIf5pKyjhh7DeE7q-t
CODEN JCLBA3
CitedBy_id crossref_primary_10_1016_S0959_8049_05_80021_8
crossref_primary_10_1242_jcs_209619
crossref_primary_10_1002_ijc_2910640407
crossref_primary_10_1007_BF02630936
crossref_primary_10_1177_030089160108700629
crossref_primary_10_1016_0009_8981_93_90158_Z
crossref_primary_10_1002_ijc_2910470603
crossref_primary_10_1016_j_yjmcc_2016_01_011
crossref_primary_10_1078_0171_9335_00283
crossref_primary_10_1177_00220345940730061001
crossref_primary_10_1016_j_exer_2017_08_017
crossref_primary_10_1089_105072503768499662
crossref_primary_10_1002_ijc_2910590507
crossref_primary_10_1016_S0006_291X_89_80034_8
crossref_primary_10_1158_1535_7163_MCT_13_0684
crossref_primary_10_1038_sj_bjc_6690809
crossref_primary_10_1002__SICI_1097_0142_19971215_80_12__2378__AID_CNCR7_3_0_CO_2_7
crossref_primary_10_1016_0014_5793_93_80479_E
crossref_primary_10_1016_0012_1606_90_90114_X
crossref_primary_10_1111_exd_12697
crossref_primary_10_1002_pmic_200300395
crossref_primary_10_1016_j_addr_2015_11_014
crossref_primary_10_1002__SICI_1098_2795_199603_43_3_392__AID_MRD14_3_0_CO_2_3
crossref_primary_10_1167_iovs_19_27171
crossref_primary_10_1038_sj_bjc_6603806
crossref_primary_10_1074_jbc_M111_303131
crossref_primary_10_1007_BF02899277
crossref_primary_10_1097_00000478_199811000_00010
crossref_primary_10_1074_jbc_M109_025924
crossref_primary_10_1002__SICI_1098_2264_199706_19_2_97__AID_GCC5_3_0_CO_2_V
crossref_primary_10_1111_jdv_20163
crossref_primary_10_1006_excr_1997_3868
crossref_primary_10_1016_j_pep_2012_12_002
crossref_primary_10_1002_cmdc_200600181
crossref_primary_10_1371_journal_pone_0148103
crossref_primary_10_1007_s00259_009_1096_y
crossref_primary_10_1002_ijc_10662
crossref_primary_10_1016_S0344_0338_11_80655_2
crossref_primary_10_1007_BF00666044
crossref_primary_10_1002_ijc_20952
crossref_primary_10_1038_s41573_023_00709_2
crossref_primary_10_1016_S0002_9440_10_64446_X
crossref_primary_10_1016_S0021_9258_19_69545_1
crossref_primary_10_1111_j_1440_1711_2005_01318_x
crossref_primary_10_1016_j_jprot_2010_05_017
crossref_primary_10_1177_002215540004800714
crossref_primary_10_1158_1541_7786_MCR_08_0195
crossref_primary_10_1016_j_lungcan_2008_07_013
crossref_primary_10_1007_s40336_021_00477_2
crossref_primary_10_1242_jcs_111_17_2655
crossref_primary_10_1016_S0964_1955_96_00074_7
crossref_primary_10_1002_ijc_2910610106
crossref_primary_10_1158_1078_0432_CCR_10_1420
crossref_primary_10_1016_0014_4827_92_90466_L
crossref_primary_10_1016_j_jbiotec_2018_12_004
crossref_primary_10_1111_j_1699_0463_1994_tb05222_x
crossref_primary_10_1054_bjom_2001_0702
crossref_primary_10_1016_j_ijom_2006_01_029
crossref_primary_10_1089_thy_2009_0332
crossref_primary_10_1002_adhm_201300597
crossref_primary_10_1158_1078_0432_CCR_06_0210
crossref_primary_10_1002__SICI_1097_0215_19980529_76_5_767__AID_IJC24_3_0_CO_2
crossref_primary_10_1016_S0945_053X_05_80026_5
crossref_primary_10_1038_nbt1197_1271
crossref_primary_10_1111_j_1432_1033_1992_tb16811_x
crossref_primary_10_1016_S0959_8049_98_00041_0
crossref_primary_10_1126_scitranslmed_3006221
crossref_primary_10_1016_j_nbt_2019_04_002
crossref_primary_10_1016_S0021_9258_18_35923_4
crossref_primary_10_1016_S0046_8177_99_90095_3
crossref_primary_10_3109_15419069509081278
crossref_primary_10_1002_ijc_32603
crossref_primary_10_1186_1755_1536_4_21
crossref_primary_10_1016_0014_5793_90_80664_5
crossref_primary_10_1186_s12885_020_07055_1
crossref_primary_10_1002_aja_1001950102
crossref_primary_10_1002_ijc_2910470512
crossref_primary_10_1242_jcs_104_3_783
crossref_primary_10_1007_s10585_015_9754_x
crossref_primary_10_18632_oncotarget_27772
crossref_primary_10_1517_14728222_9_3_491
crossref_primary_10_1074_jbc_270_15_8650
crossref_primary_10_1139_o96_091
crossref_primary_10_1038_s44321_024_00034_0
crossref_primary_10_1002__SICI_1097_0142_19971215_80_12__2484__AID_CNCR20_3_0_CO_2_7
crossref_primary_10_1161_01_ATV_17_5_1003
crossref_primary_10_1002_path_2833
crossref_primary_10_1186_s40364_023_00504_6
crossref_primary_10_1593_neo_101720
crossref_primary_10_1126_science_275_5299_547
crossref_primary_10_1016_j_addr_2017_05_011
crossref_primary_10_1016_S0003_9969_98_00035_1
crossref_primary_10_1016_j_leukres_2015_04_005
crossref_primary_10_1016_0363_5023_94_90057_4
crossref_primary_10_1007_BF02473239
crossref_primary_10_1002_1096_9896_2000_9999_9999___AID_PATH783_3_0_CO_2_P
crossref_primary_10_1136_hrt_75_4_358
crossref_primary_10_1016_j_bbcan_2020_188354
crossref_primary_10_1128_mcb_14_5_3085_3093_1994
crossref_primary_10_1006_bbrc_2001_4264
crossref_primary_10_1186_1472_6750_11_104
crossref_primary_10_1097_00005537_200307000_00023
crossref_primary_10_1016_0014_5793_93_81494_K
crossref_primary_10_1016_S0021_9258_19_47377_8
crossref_primary_10_1128_mcb_10_2_662_671_1990
crossref_primary_10_1002_0471143030_cb1005s60
crossref_primary_10_1038_nrc1627
crossref_primary_10_1083_jcb_139_1_295
crossref_primary_10_1186_1471_2407_6_8
crossref_primary_10_1177_002215549704500409
crossref_primary_10_1158_1535_7163_MCT_22_0099
crossref_primary_10_1002__SICI_1097_0215_19961104_68_3_397__AID_IJC20_3_0_CO_2_4
crossref_primary_10_1016_0014_4827_92_90100_M
crossref_primary_10_1016_j_ydbio_2007_07_005
crossref_primary_10_1016_j_addr_2005_11_002
crossref_primary_10_1111_j_1432_1033_1992_tb16813_x
crossref_primary_10_1002_pmic_200300689
crossref_primary_10_1002_ijc_2910460406
crossref_primary_10_1007_BF01464328
crossref_primary_10_1126_scitranslmed_abb2311
crossref_primary_10_1002_ijc_2910590104
crossref_primary_10_1016_S0021_9258_18_41717_6
crossref_primary_10_1038_nbt0302_264
crossref_primary_10_1111_j_1601_0825_1998_tb00282_x
crossref_primary_10_1158_1078_0432_CCR_1004_0015
crossref_primary_10_1016_j_imbio_2009_06_005
crossref_primary_10_1002_path_2626
crossref_primary_10_1002_ijc_2910580405
crossref_primary_10_1093_humrep_der195
crossref_primary_10_1182_blood_2003_04_1039
crossref_primary_10_1007_BF00268932
crossref_primary_10_1111_j_1365_2249_1994_tb06560_x
crossref_primary_10_1016_S0304_3835_98_00079_2
crossref_primary_10_1006_excr_2000_4869
crossref_primary_10_1016_j_ejca_2021_03_038
crossref_primary_10_1038_jid_2012_376
crossref_primary_10_1002_ijc_24473
crossref_primary_10_1158_1078_0432_CCR_05_0389
crossref_primary_10_1158_1078_0432_CCR_05_2448
crossref_primary_10_1046_j_1523_1755_1998_00009_x
crossref_primary_10_1007_s00438_017_1372_7
crossref_primary_10_1007_BF02389692
crossref_primary_10_1242_jcs_109_12_2895
crossref_primary_10_1111_j_1749_6632_1990_tb17924_x
crossref_primary_10_1080_00016480252814298
crossref_primary_10_1016_S0168_3659_97_00038_2
crossref_primary_10_1006_excr_1998_3946
crossref_primary_10_1016_0955_0674_89_90065_3
crossref_primary_10_1016_S0002_9394_02_01839_1
crossref_primary_10_1242_jcs_112_10_1449
crossref_primary_10_1111_j_1750_3639_2005_tb00117_x
crossref_primary_10_1007_BF02365437
crossref_primary_10_1016_j_uct_2006_05_001
crossref_primary_10_1038_ki_1996_391
crossref_primary_10_1016_0922_3371_90_90056_3
crossref_primary_10_1016_j_preteyeres_2004_02_003
crossref_primary_10_1016_S0002_9440_10_64320_9
crossref_primary_10_1002_hed_21243
crossref_primary_10_1016_S0143_4004_97_80089_6
crossref_primary_10_1111_j_1699_0463_1992_tb04005_x
crossref_primary_10_1002__SICI_1098_1136_199707_20_3_231__AID_GLIA7_3_0_CO_2_A
crossref_primary_10_1242_jcs_103_2_423
crossref_primary_10_1016_S0049_3848_10_70033_5
crossref_primary_10_1007_s10456_013_9391_4
crossref_primary_10_1038_ki_1993_9
crossref_primary_10_1016_S0960_8966_96_00391_4
crossref_primary_10_1096_fj_10_163022
crossref_primary_10_1016_S0143_4004_97_80079_3
crossref_primary_10_1007_BF02890411
crossref_primary_10_1016_j_vaccine_2011_11_010
crossref_primary_10_1016_j_jbiotec_2014_06_017
crossref_primary_10_1097_00007890_200006270_00001
crossref_primary_10_1002_ddr_10161
crossref_primary_10_1038_s41467_017_01846_0
crossref_primary_10_1039_C6TB02008A
crossref_primary_10_1371_journal_pone_0082878
crossref_primary_10_1111_j_1349_7006_1999_tb00750_x
crossref_primary_10_1016_0378_1119_94_00778_Q
crossref_primary_10_1517_14712598_2_4_371
crossref_primary_10_1006_bbrc_1999_0881
crossref_primary_10_1002_iub_493
crossref_primary_10_1242_dev_120_12_3657
crossref_primary_10_3109_15419069609010766
crossref_primary_10_1002_ijc_21677
crossref_primary_10_1016_S0940_9602_96_80102_6
crossref_primary_10_1016_j_jsb_2021_107696
crossref_primary_10_3109_15419069609010767
crossref_primary_10_1016_0003_2697_91_90551_4
crossref_primary_10_3390_cancers11091232
crossref_primary_10_1182_blood_V99_5_1659
crossref_primary_10_1016_S0021_9258_18_98735_1
crossref_primary_10_1074_jbc_274_9_5919
crossref_primary_10_18632_oncotarget_27726
crossref_primary_10_1016_S0046_8177_97_90284_7
crossref_primary_10_1038_nrclinonc_2009_52
crossref_primary_10_1016_j_oraloncology_2004_07_003
crossref_primary_10_1002__SICI_1097_0142_19980101_82_1_152__AID_CNCR19_3_0_CO_2_1
crossref_primary_10_1016_0092_8674_89_90294_8
crossref_primary_10_1016_S0002_9440_10_65388_6
crossref_primary_10_1016_j_addr_2014_07_011
crossref_primary_10_1002__SICI_1096_9896_199906_188_2_163__AID_PATH335_3_0_CO_2_1
crossref_primary_10_1182_blood_2008_06_160416
crossref_primary_10_1007_BF00941763
crossref_primary_10_1242_jcs_100_3_605
crossref_primary_10_1074_jbc_274_7_4400
crossref_primary_10_1074_jbc_M609211200
crossref_primary_10_1083_jcb_142_3_873
crossref_primary_10_1016_j_biopha_2017_07_160
crossref_primary_10_1002_ijc_2910540112
crossref_primary_10_1016_S0169_409X_97_00093_8
crossref_primary_10_1046_j_1365_2567_2000_00995_x
crossref_primary_10_3109_01913129509014601
crossref_primary_10_1016_S0169_409X_01_00190_9
crossref_primary_10_1016_S0006_8993_97_00901_3
crossref_primary_10_1016_S0021_9258_18_35819_8
crossref_primary_10_1046_j_1439_0272_2002_00455_x
crossref_primary_10_1093_rheumatology_38_11_1099
crossref_primary_10_1016_0012_1606_92_90291_N
ContentType Journal Article
Copyright Copyright 1989 The Rockefeller University Press
1989 INIST-CNRS
Copyright_xml – notice: Copyright 1989 The Rockefeller University Press
– notice: 1989 INIST-CNRS
DBID IQODW
CGR
CUY
CVF
ECM
EIF
NPM
AAYXX
CITATION
7TM
8FD
FR3
P64
RC3
7X8
5PM
DOI 10.1083/jcb.108.3.1139
DatabaseName Pascal-Francis
Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
CrossRef
Nucleic Acids Abstracts
Technology Research Database
Engineering Research Database
Biotechnology and BioEngineering Abstracts
Genetics Abstracts
MEDLINE - Academic
PubMed Central (Full Participant titles)
DatabaseTitle MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
CrossRef
Genetics Abstracts
Engineering Research Database
Technology Research Database
Nucleic Acids Abstracts
Biotechnology and BioEngineering Abstracts
MEDLINE - Academic
DatabaseTitleList
CrossRef
MEDLINE

Genetics Abstracts
MEDLINE - Academic
Database_xml – sequence: 1
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 2
  dbid: EIF
  name: MEDLINE
  url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search
  sourceTypes: Index Database
DeliveryMethod fulltext_linktorsrc
Discipline Biology
EISSN 1540-8140
EndPage 1148
ExternalDocumentID 10_1083_jcb_108_3_1139
2646306
7196557
1613403
Genre Research Support, Non-U.S. Gov't
Journal Article
GroupedDBID ---
-DZ
-~X
.55
.GJ
0VX
123
18M
1VV
29K
2WC
36B
3O-
4.4
53G
85S
9QQ
AAUTI
ABDNZ
ABOCM
ABPIV
ABPPZ
ABRJW
ABZEH
ACGFO
ACGOD
ACIWK
ACKIV
ACKOT
ACNCT
ACNKL
ACPRK
ACPVT
ADBBV
AENEX
AEUPB
AFFDN
AFMIJ
AFNDN
AFOSN
AFRAH
AGHSJ
AHJTV
AI.
ALMA_UNASSIGNED_HOLDINGS
AOIJS
B-7
BAWUL
BTFSW
C1A
C45
CS3
D-I
D0L
DIK
DU5
E3Z
EBS
EJD
F20
F5P
F9R
FRP
GX1
HF~
HGD
HYE
IH2
J5H
JENOY
JST
JZ9
KQ8
MVM
N9A
O5R
O5S
OK1
P2P
PQQKQ
R.V
RHF
RHI
RNS
RPM
RXW
SJN
TN5
TR2
TRP
TWZ
UHB
UKR
UPT
VH1
VQA
W8F
WH7
WOQ
X7L
X7M
XOL
YNH
YOC
YQT
YYP
YZZ
ZA5
ZCA
ZGI
~KM
08R
34G
39C
AAUGY
ABPTK
ABTAH
ACYGS
AGCDD
AGJRR
BKOMP
EMB
EMOBN
H13
IQODW
NHB
OVD
SV3
TAE
TAF
UBX
YKV
YSK
YWH
ZY4
AEILP
CGR
CUY
CVF
ECM
EIF
NPM
AAYXX
CITATION
7TM
8FD
FR3
P64
RC3
7X8
5PM
ID FETCH-LOGICAL-c466t-6412bba9524b1f0549534e3001f7345dfec26edb2585557d0b34fe6aa70cb6b23
IEDL.DBID RPM
ISSN 0021-9525
IngestDate Tue Sep 17 20:49:26 EDT 2024
Fri Oct 25 09:37:58 EDT 2024
Fri Oct 25 22:07:33 EDT 2024
Thu Sep 26 18:52:38 EDT 2024
Sat Sep 28 08:36:44 EDT 2024
Sun Oct 29 17:06:44 EDT 2023
Fri Feb 02 07:01:33 EST 2024
IsDoiOpenAccess true
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue 3
Keywords Characterization
Human
Immunohistochemistry
Tissue
Production
Glycoproteins
Monoclonal antibody
Gene expression
Localization
Fibronectin
Aminoacid sequence
Isomer
Language English
License CC BY 4.0
This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c466t-6412bba9524b1f0549534e3001f7345dfec26edb2585557d0b34fe6aa70cb6b23
Notes ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ObjectType-Article-1
ObjectType-Feature-2
OpenAccessLink https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2115391/
PMID 2646306
PQID 15366233
PQPubID 23462
PageCount 10
ParticipantIDs pubmedcentral_primary_oai_pubmedcentral_nih_gov_2115391
proquest_miscellaneous_78864000
proquest_miscellaneous_15366233
crossref_primary_10_1083_jcb_108_3_1139
pubmed_primary_2646306
pascalfrancis_primary_7196557
jstor_primary_1613403
PublicationCentury 1900
PublicationDate 1989-03-01
PublicationDateYYYYMMDD 1989-03-01
PublicationDate_xml – month: 03
  year: 1989
  text: 1989-03-01
  day: 01
PublicationDecade 1980
PublicationPlace New York, NY
PublicationPlace_xml – name: New York, NY
– name: United States
PublicationTitle The Journal of cell biology
PublicationTitleAlternate J Cell Biol
PublicationYear 1989
Publisher Rockefeller University Press
The Rockefeller University Press
Publisher_xml – name: Rockefeller University Press
– name: The Rockefeller University Press
SSID ssj0004743
Score 1.8205285
Snippet Fibronectin (FN) represents the mixture of a number of structurally different molecules (isoforms) whose make-up varies depending on the FN sources. FN from...
The authors have recently demonstrated that the B-FN isoform derives from a differential splicing pattern of the FN primary transcript which leads, in...
SourceID pubmedcentral
proquest
crossref
pubmed
pascalfrancis
jstor
SourceType Open Access Repository
Aggregation Database
Index Database
Publisher
StartPage 1139
SubjectTerms Alternative splicing
Analytical, structural and metabolic biochemistry
Antibodies, Monoclonal
Biological and medical sciences
Biotechnology
Cell Line
Cell lines
Cells
Epitopes
Exons
Female
Fetus
Fetus - analysis
Fibroblasts
Fibronectins - analysis
Fibronectins - genetics
Fibronectins - immunology
Fundamental and applied biological sciences. Psychology
Glycoproteins
Health. Pharmaceutical industry
Humans
Immunoenzyme Techniques
Industrial applications and implications. Economical aspects
Messenger RNA
Miscellaneous
Monoclonal antibodies
Myometrium - analysis
Neoplasms - analysis
Ovary - analysis
Production of active biomolecules
Protein isoforms
Proteins
RNA Precursors - genetics
RNA Splicing
Synovial Membrane - analysis
Tumor cell line
Tumors
Title A Tumor-Associated Fibronectin Isoform Generated by Alternative Splicing of Messenger RNA Precursors
URI https://www.jstor.org/stable/1613403
https://www.ncbi.nlm.nih.gov/pubmed/2646306
https://search.proquest.com/docview/15366233
https://search.proquest.com/docview/78864000
https://pubmed.ncbi.nlm.nih.gov/PMC2115391
Volume 108
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Nb9QwEB21lSr1gii0ItAWH5A4ZTeJv5LjqqIqoFYIUam3yE5sWNRNVpvdQ_89M06ydBFcuCVyEkV-E_uN_fIG4B0XZCJWZbFVuoiFTDmOg3WFgNTkB4XDYUgUb27V9Z34dC_v90CO_8IE0X5l55PmYTFp5j-CtnK5qKajTmz65eYSkxbJi3S6D_sYoGOKPv4MqQepHGkPZCZHp8acT39Wlg4mnCqZFEdwiGxAcSp29GRO6mWJpJE0HXaT7-tb_I2A_qmjfDIxXT2HZwOjZLP-zY9hzzUv4LCvMfn4EuoZW28W7So2AxCuZh5z5Lahoa5h864l3sq-B_9parWPLOyhN8ETnHW0w40THGs9W5DTOK0Dsq-3M7Zc0WJ91666E7i7-vDt8joeSivElVBqHSuRZtYa7B9hU4-0rZBcOI5zltcIX-1dlSlX2wyzCSl1nVguvFPG6KSyymb8FA4afNFXwPB-kyhvee4S4aSyBs914X3O09SoLIL3Y-eWy95Boww73zkvERA6KHlJgERwEvr-92XINUTCIzjfwWLbrskOUeoI3o7YlPhx0I6HaVy76UoME4X8jv_7Cp3nCoexJILTHsvtw4fYiEDvgLxtJ1_u3RYM1-DPPYTn6_--8w0c9VI2krqdwcF6tXHnyH3W9gJZ_8fPFyHifwE0_QUh
link.rule.ids 230,315,730,783,787,888,27938,27939,53806,53808
linkProvider National Library of Medicine
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3Pb5RAFH6pNY29-LNNqdbOwcQTLDC_4LhpbFbtboxpTW-EgZm66sJmYQ_1r_fNAGu30YPeIA8I8L2ZeY_38T2AN5RZEbEi9pWQqc94RHEeLAsEpLR6UDgdukRxOhOTK_bhml_vAB_-hXGk_ULNg-rHIqjmXx23crkoRgNPbPRpeoZJC6dpNHoAD3G8hmJI0offIWVPlrPsAx7zQasxoaNvhbIbAbW9TNJ92MN4QFDb7ujOqtQREy1LMm_wRZmuw8WfQtD7TMo7S9P5E_gyPFTHSPkerFsVFD_v6T3-81M_hcd9sErGnfkZ7OjqOex17StvX0A5Ju16Ua_8vMdYl8Rg-l1XdhatyLypbUhMbpy0tbWqW-LK85WTGyeNLZ7j2klqQxZWxNx-YiSfZ2OyXNk6QFOvmgO4On93eTbx-64NfsGEaH3BolipHF88U5HBiDDllGmKy6GR6Bml0UUsdKliTFQ4l2WoKDNa5LkMCyVUTA9ht8IbPQKC5-ehMIomOmSaC5XjvkyNSWgU5SL24O2AWrbsxDkyV1RPaIZI242MZhZpDw4cqL8PwzCGhdSDky2QN3ZplRa59OB0AD3DcWeLKXml63WTIRYCQ0f69yNkkgicIUMPDjsn2Vy8dzoP5Jb3bOxW8nvbgj7hpL97Hzj-7zNP4dHkcnqRXbyffXwJ-x1jzjLqXsFuu1rrEwyxWvXaDahfIW8mFg
linkToPdf http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lb9QwEB5BUVEvPFsRoNQHJE55-pUcV4VVeXRVISpVXKI4sWGBTVab7KH8esZOsuxWcOktkZ0ozje2ZzJfvgF4TZkVESsTXwmZ-YzHFNfBqkRAKqsHhcuhCxTPZ-Lskn244ldbpb4cab9U86D-tQjq-XfHrVwuynDkiYUX56cYtHCaxeGyMuFduIdzNkrHQH38JVIOhDnLQOAJH_UaUxr-KJU9CKitZ5IdwD76BILakkdbO1NPTrRMyaLFl2X6Khf_ckNvsim3tqfpQ_g6DqxnpfwM1p0Kyt83NB9vNfJH8GBwWsmk7_IY7uj6Cez3ZSyvn0I1Id160az8YsBaV8RgGN7UdjWtybxtrGtMvjmJa9uqrolL09dOdpy0NomOeyhpDFlYMXP7qZF8nk3IcmXzAW2zag_hcvruy-mZP1Rv8EsmROcLFidKFfjymYoNeoYZp0xT3BaNRAupjC4ToSuVYMDCuawiRZnRoihkVCqhEnoEezU-6DMgeH0RCaNoqiOmuVAFnsvMmJTGcSESD96MyOXLXqQjd8n1lOaItj3IaW7R9uDQAfu3G7ozLKIeHO8AvWmXVnGRSw9ORuBznH82qVLUulm3OeIh0IWk_-8h01TgShl5cNQbyubmg-F5IHcsaNNupb93W9AunAT4YAfPb33lCdy_eDvNP72ffXwBBz1xzhLrXsJet1rrY_S0OvXKzak_P6wolg
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=A+tumor-associated+fibronectin+isoform+generated+by+alternative+splicing+of+messenger+RNA+precursors&rft.jtitle=The+Journal+of+cell+biology&rft.au=Carnemolla%2C+B&rft.au=Balza%2C+E&rft.au=Siri%2C+A&rft.au=Zardi%2C+L&rft.date=1989-03-01&rft.issn=0021-9525&rft.volume=108&rft.issue=3&rft.spage=1139&rft_id=info:doi/10.1083%2Fjcb.108.3.1139&rft_id=info%3Apmid%2F2646306&rft.externalDocID=2646306
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0021-9525&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0021-9525&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0021-9525&client=summon