Severe developmental timing defects in the prothoracicotropic hormone (PTTH)-deficient silkworm, Bombyx mori

• Published in: Insect biochemistry and molecular biology Vol. 87; pp. 14 - 25
• Main Authors: Uchibori-Asano, Miwa, Kayukawa, Takumi, Sezutsu, Hideki, Shinoda, Tetsuro, Daimon, Takaaki
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.08.2017
• Subjects: Animals; Bombyx - genetics; Bombyx - growth & development; Bombyx - metabolism; Ecdysone; Ecdysone - biosynthesis; Endocrinology; Genome editing; Hemolymph - chemistry; Insect Hormones - deficiency; Insect Hormones - genetics; Larva - growth & development; Larva - metabolism; Metamorphosis; Metamorphosis, Biological - physiology; Molting - physiology; Pupa - growth & development; Pupa - metabolism; RNA, Messenger - metabolism; Signal Transduction; Steroidogenesis; Genome editing; Steroidogenesis; Ecdysone; Metamorphosis; Endocrinology
• ISSN: 0965-1748; 1879-0240
• DOI: 10.1016/j.ibmb.2017.06.007

The insect neuropeptide prothoracicotropic hormone (PTTH) triggers the biosynthesis and release of the molting hormone ecdysone in the prothoracic gland (PG), thereby controlling the timing of molting and metamorphosis. Despite the well-documented physiological role of PTTH and its signaling pathway in the PG, it is not clear whether PTTH is an essential hormone for ecdysone biosynthesis and development. To address this question, we established and characterized a PTTH knockout line in the silkworm, Bombyx mori. We found that PTTH knockouts showed a severe developmental delay in both the larval and pupal stages. Larval phenotypes of PTTH knockouts can be classified into three major classes: (i) developmental arrest during the second larval instar, (ii) precocious metamorphosis after the fourth larval instar (one instar earlier in comparison to the control strain), and (iii) metamorphosis to normal-sized pupae after completing the five larval instar stages. In PTTH knockout larvae, peak levels of ecdysone titers in the hemolymph were dramatically reduced and the timing of peaks was delayed, suggesting that protracted larval development is a result of the reduced and delayed synthesis of ecdysone in the PG. Despite these defects, low basal levels of ecdysone were maintained in PTTH knockout larvae, suggesting that the primary role of PTTH is to upregulate ecdysone biosynthesis in the PG during molting stages, and low basal levels of ecdysone can be maintained in the absence of PTTH. We also found that mRNA levels of genes involved in ecdysone biosynthesis and ecdysteroid signaling pathways were significantly reduced in PTTH knockouts. Our results provide genetic evidence that PTTH is not essential for development, but is required to coordinate growth and developmental timing. [Display omitted] •Insect PTTH activates the biosynthesis of the molting hormone ecdysone.•PTTH knockout silkworms exhibit severe defects in ecdysone biosynthesis and developmental timing.•mRNA levels of ecdysone biosynthetic genes were dramatically reduced in PTTH knockouts.•PTTH is required to coordinate growth and developmental timing.