Modulation of Circadian Gene Expression and Metabolic Compensation by the RCO-1 Corepressor of Neurospora crassa

• Published in: Genetics (Austin) Vol. 204; no. 1; pp. 163 - 176
• Main Authors: Olivares-Yañez, Consuelo, Emerson, Jillian, Kettenbach, Arminja, Loros, Jennifer J, Dunlap, Jay C, Larrondo, Luis F
• Format: Journal Article
• Language: English
• Published: United States Genetics Society of America 01.09.2016
• Subjects: Circadian Clocks - genetics; Circadian Clocks - physiology; Circadian rhythm; Circadian Rhythm - genetics; Compensation; Defects; Fungal Proteins - biosynthesis; Fungal Proteins - genetics; Fungal Proteins - metabolism; Gene expression; Gene Expression Regulation, Fungal; Glucose; Investigations; Kinases; Metabolism; Mutation; Neurospora; Neurospora crassa; Neurospora crassa - genetics; Neurospora crassa - metabolism; Phosphorylation; Repressor Proteins - biosynthesis; Repressor Proteins - genetics; Repressor Proteins - metabolism; Saccharomyces cerevisiae; Neurospora crassa; core-clock mechanism; circadian clocks; corepressor; frequency
• ISSN: 1943-2631; 0016-6731; 1943-2631
• DOI: 10.1534/genetics.116.191064

Neurospora crassa is a model organism for the study of circadian clocks, molecular machineries that confer ∼24-hr rhythms to different processes at the cellular and organismal levels. The FREQUENCY (FRQ) protein is a central component of the Neurospora core clock, a transcription/translation negative feedback loop that controls genome-wide rhythmic gene expression. A genetic screen aimed at determining new components involved in the latter process identified regulation of conidiation 1 (rco-1), the ortholog of the Saccharomyces cerevisiae Tup1 corepressor, as affecting period length. By employing bioluminescent transcriptional and translational fusion reporters, we evaluated frq and FRQ expression levels in the rco-1 mutant background observing that, in contrast to prior reports, frq and FRQ expression are robustly rhythmic in the absence of RCO-1, although both amplitude and period length of the core clock are affected. Moreover, we detected a defect in metabolic compensation, such that high-glucose concentrations in the medium result in a significant decrease in period when RCO-1 is absent. Proteins physically interacting with RCO-1 were identified through co-immunoprecipitation and mass spectrometry; these include several components involved in chromatin remodeling and transcription, some of which, when absent, lead to a slight change in period. In the aggregate, these results indicate a dual role for RCO-1: although it is not essential for core-clock function, it regulates proper period and amplitude of core-clock dynamics and is also required for the rhythmic regulation of several clock-controlled genes.