miR-590 promotes cell proliferation and invasion in T-cell acute lymphoblastic leukaemia by inhibiting RB1

• Published in: Oncotarget Vol. 7; no. 26; pp. 39527 - 39534
• Main Authors: Miao, Mei-hua, Ji, Xue-qiang, Zhang, Hao, Xu, Jun, Zhu, Hong, Shao, Xue-jun
• Format: Journal Article
• Language: English
• Published: United States Impact Journals LLC 28.06.2016
• Subjects: 3' Untranslated Regions; Adolescent; Adult; Cadherins - metabolism; Cell Cycle; Cell Movement; Cell Proliferation; Child; Child, Preschool; Female; Gene Expression Regulation, Leukemic; Humans; Immunophenotyping; Infant; Male; Matrix Metalloproteinase 9 - metabolism; MicroRNAs - metabolism; Neoplasm Invasiveness; Precursor T-Cell Lymphoblastic Leukemia-Lymphoma - genetics; Precursor T-Cell Lymphoblastic Leukemia-Lymphoma - immunology; Research Paper; Retinoblastoma Binding Proteins - antagonists & inhibitors; Retinoblastoma Binding Proteins - metabolism; Ubiquitin-Protein Ligases - antagonists & inhibitors; Ubiquitin-Protein Ligases - metabolism; Young Adult; RB1; miR-590; cancer; T-ALL
• ISSN: 1949-2553; 1949-2553
• DOI: 10.18632/oncotarget.8414

MicroRNAs play important roles in the pathogenesis of cancers by inhibiting gene expression at posttranscriptional level. Here, we identified that miR-590 and its predicted target gene RB1 are differentially expressed in T-cell acute lymphoblastic leukaemia (T-ALL). The correlation between miR-590 and RB1 was further confirmed in 395 T-ALL patients. In T-ALL cell lines, miR-590 promoted the cell proliferation by increasing G1/S transition. Moreover, migration and invasion assay showed that miR-590 promotes the migration and invasion of T-ALL cells by increasing E-cadherin and inhibiting MMP-9. Luciferase assays confirmed that miR-590 directly binds to the 3'untranslated region of RB1, and western blotting showed that miR-590 suppresses the expression of RB1 at the protein levels. This study indicated that miR-590 inhibits RB1 and promotes proliferation and invasion of T-ALL cells. Thus, miR-590 may represent a potential therapeutic target for T-ALL intervention.