Calcium dynamics and modulation in carrot somatic embryogenesis

Free calcium (Ca ) is a pivotal player in different and morphogenic processes. In the induction of somatic embryogenesis, its role has been demonstrated in different species. In carrot, however, this role has been more controversial. In this work, we developed carrot lines expressing Ca sensors. Wit...

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Published inFrontiers in plant science Vol. 14; p. 1150198
Main Authors Calabuig-Serna, Antonio, Mir, Ricardo, Arjona, Paloma, Seguí-Simarro, Jose María
Format Journal Article
LanguageEnglish
Published Switzerland Frontiers Media S.A 31.03.2023
Subjects
callose
DAUCUS carota
EGTA
FRET
in vitro culture
ionophore A23187
Plant Science
W-7
callose
DAUCUS carota
morphogenesis
in vitro culture
FRET
ionophore A23187
EGTA
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Summary:Free calcium (Ca ) is a pivotal player in different and morphogenic processes. In the induction of somatic embryogenesis, its role has been demonstrated in different species. In carrot, however, this role has been more controversial. In this work, we developed carrot lines expressing Ca sensors. With them, Ca levels and distribution in the different embryogenic structures formed during the induction and development of somatic embryos were analyzed by FRET. We also used different chemicals to modulate intracellular Ca levels (CaCl , ionophore A23187, EGTA), to inhibit calmodulin (W-7) and to inhibit callose synthesis (2-deoxy-D-glucose) at different times, principally during the first stages of embryo induction. Our results showed that high Ca levels and the development of a callose layer are markers of cells induced to embryogenesis, which are the precursors of somatic embryos. Disorganized calli and embryogenic masses have different Ca patterns associated to their embryogenic competence, with higher levels in embryogenic cells than in callus cells. The efficiency of somatic embryogenesis in carrot can be effectively modulated by allowing, within a range, more Ca to enter the cell to act as a second messenger to trigger embryogenesis induction. Once induced, Ca -calmodulin signaling seems related with the transcriptional remodeling needed for embryo progression, and alterations of Ca or calmodulin levels negatively affect the efficiency of the process.
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Reviewed by: Claudia Renate Stange, University of Chile, Chile; Tatsuhito Fujimura, University of Tsukuba, Japan
Edited by: Nuria Alburquerque, Center for Edaphology and Applied Biology of Segura (CSIC), Spain
This article was submitted to Plant Biotechnology, a section of the journal Frontiers in Plant Science
ISSN:1664-462X
1664-462X
DOI:10.3389/fpls.2023.1150198