Developmental expression of the Arabidopsis cyclin gene cyc1At

• Published in: The Plant cell Vol. 6; no. 12; pp. 1763 - 1774
• Main Authors: Paulo C. G. Ferreira, Hemerly, Adriana S., Janice de Almeida Engler, Van Montagu, Marc, Engler, Gilbert, Inzé, Dirk
• Format: Journal Article
• Language: English
• Published: United States American Society of Plant Physiologists 01.12.1994
• Subjects: 6 benziladenina; 6 benzyladenine; acide naphtalene acetique; acido naftilacetico; adn recombinado; adn recombine; amidas; amide; amides; apical meristems; Arabidopsis - embryology; Arabidopsis - genetics; arabidopsis thaliana; Base Sequence; Cell cycle; Cell Cycle - genetics; Cell division; Cyclins; Cyclins - genetics; division cellulaire; division celular; Embryos; expresion genica; expression des genes; gene; gene expression; Gene Expression Regulation, Developmental; Gene Expression Regulation, Plant; Genes; genetica; genetics; genetique; glicosidasas; glycosidase; glycosidases; In situ hybridization; meristemas; meristemas apicales; meristeme; meristeme apical; Meristems; Molecular Sequence Data; naa; nicotiana tabacum; Oligodeoxyribonucleotides; orizalina; oryzalin; Plant cells; Plant Growth Regulators - physiology; Plant roots; Plants; proteinas; proteine; proteins; racine; raices; recombinant dna; roots
• ISSN: 1040-4651; 1532-298X
• DOI: 10.1105/tpc.6.12.1763

In eukaryotes, the control of cell cycle progression is exercised by heteromeric protein kinase complexes composed of a cell cycle-dependent, kinase-related subunit (Cdc2) and a cyclin subunit. To explore the possibility that cyclin transcription plays a role in the developmental regulation of ceil division, we examined the spatial and temporal expression of a cyclin gene (cyc1At) in Arabidopsis. In root and shoot apical meristems and during embryogenesis, cyc1At expression is almost exclusively confined to dividing cells. A cell-specific pattern of cyc1At expression was noticed in root meristems. We examined the effects of induction of cell division of differentiated cells on cyc1At expression. During lateral root formation, induction of cyc1At expression is a very early event and was detected before anatomical modifications were visible. Treatment of roots with oryzalin, which blocks cell division in metaphase, did not inhibit the auxin induction of cyc1At, suggesting that induction of cyc1At expression precedes the completion of the first division cycle after induction of lateral roots. In tobacco protoplasts, an increase in cyc1At expression was observed only when cell division was induced. Together, the results suggest that Cyc1At accumulation in Arabidopsis is transcriptionally regulated and might be one of the limiting factors for the activation of cell division.