Genome-wide characterization and identification of Trihelix transcription factors and expression profiling in response to abiotic stresses in Chinese Willow ( Salix matsudana Koidz)

• Published in: Frontiers in plant science Vol. 14; p. 1125519
• Main Authors: Yang, Jie, Tang, Zhixuan, Yang, Wuyue, Huang, Qianhui, Wang, Yuqing, Huang, Mengfan, Wei, Hui, Liu, Guoyuan, Lian, Bolin, Chen, Yanhong, Zhang, Jian
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 03.03.2023
• Subjects: genome-wide characterization; Plant Science; RNA-Seq; Salix matsudana; submergence stress; Trihelix family; Salix matsudana; submergence stress; Trihelix family; RNA-Seq; genome-wide characterization
• ISSN: 1664-462X; 1664-462X
• DOI: 10.3389/fpls.2023.1125519

Trihelix transcription factors (TTF) are a class of light-responsive proteins with a typical triple-helix structure (helix-loop-helix-loop-helix). Members of this gene family play an important role in plant growth and development, especially in various abiotic stress responses. Koidz is an allotetraploid ornamental forest tree that is widely planted for its excellent resistance to stress, but no studies on its Trihelix gene family have been reported. In this study, the Trihelix gene family was analyzed at the genome-wide level in . A total of 78 Trihelix transcription factors ( ) were identified, distributed on 29 chromosomes, and classified into four subfamilies (GT-1, GT-2, SH4, SIP1) based on their structural features. The gene structures and conserved functional domains of these Trihelix genes are similar in the same subfamily and differ between subfamilies. The presence of multiple stress-responsive cis-elements on the promoter of the Trihelix gene suggests that the Trihelix gene may respond to abiotic stresses. Expression pattern analysis revealed that Trihelix genes have different functions during flooding stress, salt stress, drought stress and low temperature stress in . Given that , as a differentially expressed gene, has a faster response to flooding stress, we selected for functional studies. Overexpression of in (Arabidopsis) enhances its tolerance to flooding stress. Under flooding stress, the leaf cell activity and peroxidase activity (POD) of the overexpression strain were significantly higher than the leaf cell activity and POD of the wild type, and the malondialdehyde (MDA) content was significantly lower than the MDA content of the wild type. Thus, these results suggest that enhances plant flooding tolerance and plays a positive regulatory role in plant flooding tolerance.