Induction of inflammatory response in human cell lines by arsenic-contaminated soil-isolated bacterium Micrococcus sp. KUMAs15

• Published in: Egyptian Journal of Medical Human Genetics Vol. 20; no. 1; pp. 1 - 7
• Main Authors: Paul, Tanmoy, Mukhirji, Samir Kumar
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Egyptian Society of Human Genetics 15.08.2019; Springer Berlin Heidelberg; Springer; Springer Nature B.V; SpringerOpen
• Subjects: Adsorption; Arsenic; Bacteria; Bioremediation; Cell lines; Cells; Cytokines; Decontamination; Gene expression; Genes; Immune response; Inflammation; Interleukin 12; Interleukin 6; Interleukin 8; Medicine; Medicine & Public Health; Micrococcus; Pathogenicity; Soil microbiology; Soil microorganisms; Soil pollution; البكتيريا; التربة; الخلايا; المكورات الدقيقة; India; Pathogenicity; Cytokines
• ISSN: 1110-8630; 2090-2441
• DOI: 10.1186/s43042-019-0011-8

Background: An arsenic-resistant microbial strain, Micrococcus sp. KUMAs15 isolated from West Bengal, India, has demonstrated high resistance to arsenic due to its arsenic accumulation and adsorption ability, establishing the strain as a potential arsenic bioremediation candidate for arsenic-contaminated niche. The successful field application of the microbe necessitates evaluation of probable immunotoxin in ecological reactions on human cells. The present study determines expression profiles of pro-inflammatory and anti-inflammatory cytokines in cells exposed to KUMAsI5. Results: The present study explored the alterations in expressions of the pro-inflammatory and anti-inflammatory cytokines in two human cell lines exposed to KUMAsI5. The expression profile of the cytokine genes demonstrated that Micrococcus sp. KUMAsI5 does not significantly induce inflammatory effects in these human cell lines. The upregulated expression of IL-8 and downregulated expression of IL-6 were observed in HaCaT. The HepG2 have shewn downregulated IL-12 gene expression. These observations indicate the non-pathogenicity of KUMAsI5 on the human cell lines. Conclusion: The observations from the study extend the applicability of the arsenic-resistant Micrococcus sp. KUMAsI5 for environmental arsenic decontamination. The isolate KUMAsI5 was observed to be non-pathogenic to the human cell lines, as the strain does not initiate inflammatory reactants in these cell lines.