Isolation and HPLC method development of azafrin from Alectra parasitica var. chitrakutensis

• Published in: Natural product research Vol. 28; no. 13; pp. 940 - 944
• Main Authors: Agrawal, Poonam, Laddha, Kirti, Tiwari, Ashok
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 01.01.2014
• Subjects: Alectra; Alectra parasitica; azafrin; Carotenoids - analysis; Carotenoids - chemistry; Carotenoids - isolation & purification; Chromatography, High Pressure Liquid - methods; Chromatography, Thin Layer; HPLC; isolation; Nuclear Magnetic Resonance, Biomolecular; Plant Extracts - chemistry; Rhizome - chemistry; Scrophulariaceae; Scrophulariaceae - chemistry; Alectra parasitica; isolation; azafrin; HPLC

This study was undertaken to isolate and quantify azafrin in Alectra parasitica (Scrophulariaceae) rhizomes. A simple method for the isolation of carotenoid, azafrin, involves solvent extraction of the dried rhizome powder using a single solvent and further purification by recrystallisation. The str...