Isolation and HPLC method development of azafrin from Alectra parasitica var. chitrakutensis

This study was undertaken to isolate and quantify azafrin in Alectra parasitica (Scrophulariaceae) rhizomes. A simple method for the isolation of carotenoid, azafrin, involves solvent extraction of the dried rhizome powder using a single solvent and further purification by recrystallisation. The str...

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Published inNatural product research Vol. 28; no. 13; pp. 940 - 944
Main Authors Agrawal, Poonam, Laddha, Kirti, Tiwari, Ashok
Format Journal Article
LanguageEnglish
Published England Taylor & Francis 01.01.2014
Subjects
Alectra
Alectra parasitica
azafrin
Carotenoids - analysis
Carotenoids - chemistry
Carotenoids - isolation & purification
Chromatography, High Pressure Liquid - methods
Chromatography, Thin Layer
HPLC
isolation
Nuclear Magnetic Resonance, Biomolecular
Plant Extracts - chemistry
Rhizome - chemistry
Scrophulariaceae
Scrophulariaceae - chemistry
Alectra parasitica
isolation
azafrin
HPLC
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Summary:This study was undertaken to isolate and quantify azafrin in Alectra parasitica (Scrophulariaceae) rhizomes. A simple method for the isolation of carotenoid, azafrin, involves solvent extraction of the dried rhizome powder using a single solvent and further purification by recrystallisation. The structure of the compound was elucidated and confirmed by thin-layer chromatography, infrared spectroscopy, mass spectroscopy and nuclear magnetic resonance spectral analysis. A specific and rapid reversed-phase high-performance liquid chromatography (HPLC) method was developed for the analysis of azafrin. The method was validated for accuracy, precision, linearity and specificity. Validation revealed that the method is specific, accurate, precise, reliable and reproducible. The proposed HPLC method can be used for the identification and quantitative analysis of azafrin in A. parasitica rhizomes.
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ISSN:1478-6419
1478-6427
DOI:10.1080/14786419.2014.896009