The Clinicopathological Significance of miR-133a in Colorectal Cancer

• Published in: Disease markers Vol. 2014; no. 2014; pp. 1 - 8
• Main Authors: Ng, Lui, Lam, Colin Siu-Chi, Wan, Timothy Ming-Hun, Chow, Ariel Ka-Man, Wong, Sunny Kit-Man, Li, Hung-Sing, Man, Johnny Hon-Wai, Lo, Oswens Siu-Hung, Foo, Dominic, Cheung, Alvin, Yau, Thomas, Poon, Jensen Tung-Chung, Poon, Ronnie Tung-Ping, Law, Wai-Lun, Pang, Roberta Wen-Chi
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Puplishing Corporation 01.01.2014; Hindawi Publishing Corporation; John Wiley & Sons, Inc
• Subjects: Adaptor Proteins, Signal Transducing - genetics; Adaptor Proteins, Signal Transducing - metabolism; Adult; Aged; Aged, 80 and over; Biomarkers, Tumor - genetics; Biomarkers, Tumor - metabolism; Cancer; Carrier Proteins - genetics; Carrier Proteins - metabolism; Case-Control Studies; Caveolin 1 - genetics; Caveolin 1 - metabolism; Clinical pathology; Colorectal cancer; Colorectal Neoplasms - diagnosis; Colorectal Neoplasms - metabolism; Colorectal Neoplasms - pathology; Comparative analysis; Cytoskeletal Proteins - genetics; Cytoskeletal Proteins - metabolism; Down-Regulation; Female; Genetic aspects; Health aspects; Humans; Intestinal Mucosa - metabolism; Intestinal Mucosa - pathology; LIM Domain Proteins - genetics; LIM Domain Proteins - metabolism; Male; Microfilament Proteins - genetics; Microfilament Proteins - metabolism; MicroRNA; MicroRNAs - genetics; MicroRNAs - metabolism; Middle Aged; Oncology, Experimental; Prognosis
• ISSN: 0278-0240; 1875-8630
• DOI: 10.1155/2014/919283

This study determined the expression of microRNA-133a (MiR-133a) in colorectal cancer (CRC) and adjacent normal mucosa samples and evaluated its clinicopathological role in CRC. The expression of miR-133a in 125 pairs of tissue samples was analyzed by quantitative real-time polymerase chain reaction (qRT-PCR) and correlated with patient’s clinicopathological data by statistical analysis. Endogenous expression levels of several potential target genes were determined by qRT-PCR and correlated using Pearson’s method. MiR-133a was downregulated in 83.2% of tumors compared to normal mucosal tissue. Higher miR-133a expression in tumor tissues was associated with development of distant metastasis, advanced Dukes and TNM staging, and poor survival. The unfavorable prognosis of higher miR-133a expression was accompanied by dysregulation of potential miR-133a target genes, LIM and SH3 domain protein 1 (LASP1), Caveolin-1 (CAV1), and Fascin-1 (FSCN1). LASP1 was found to possess a negative correlation (γ=-0.23), whereas CAV1 exhibited a significant positive correlation (γ=0.27), and a stronger correlation was found in patients who developed distant metastases (γ=0.42). In addition, a negative correlation of FSCN1 was only found in nonmetastatic patients. In conclusion, miR-133a was downregulated in CRC tissues, but its higher expression correlated with adverse clinical characteristics and poor prognosis.