Temperature-induced micellar-lamellar transformation in binary mixtures of saturated phosphatidylcholines with sodium cholate

• Published in: FEBS letters Vol. 358; no. 1; pp. 17 - 22
• Main Authors: Polozova, Alla I., Dubachev, Gennady E., Simonova, Tatyana N., Barsukov, Leonid I.
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 16.01.1995
• Subjects: Calorimetry, Differential Scanning; ChNa; Cholic Acid; Cholic Acids - chemistry; Differential scanning calorimetry; dimyristoylphosphatidylcholine; dipalmitoylphosphatidylcholine; DMPC; DPPC; DSC; effective detergent/lipid molar ratio in the mixed structures; Electron microscopy; Magnetic Resonance Spectroscopy; Micellar-lamellar transition; Micelles; Microscopy, Electron; Nephelometry and Turbidimetry; NMR; Organelles - chemistry; Organelles - ultrastructure; Phosphatidylcholines - chemistry; Saturated phosphatidylcholine; Sodium cholate; Temperature; Differential scanning calorimetry; NMR; DSC; R e; ChNa; Saturated phosphatidylcholine; Sodium cholate; Electron microscopy; DMPC; Micellar-lamellar transition; DPPC
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(94)01378-E

The transition states of binary mixtures of dipalmitoyl- and dimyristoylphosphatidylcholines with sodium cholate at the reversible temperature-induced micellar-lamellar transformation were characterized by turbidimetry, electron microscopy, 31P NMR and differential scanning calorimetry. This transformation is triggered by the phospholipidacyl chain melting, and appears to include two structural pathways: (i) from discoidal mixed micelles to network-like structures composed of long interlaced rod-like micelles, then to multilayer membrane structures, and finally to multilamellar vesicles; and (ii) from discoidal micelles to membrane fragments and finally to unilamellar vesicles.