Human-papillomavirus DNA in cholesteatomas

Cholesteatoma of the middle ear is a relatively common disorder, often with severe consequences. Histologically, the aggressively growing, bone-destructing form shows papillary growth and koilocytosis, which are characteristic of papillomavirus-induced lesions. A PCR (polymerase chain reaction) meth...

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Bibliographic Details
Published inInternational journal of cancer Vol. 59; no. 4; p. 463
Main Authors Bergmann, K, Hoppe, F, He, Y, Helms, J, Müller-Hermelink, H K, Stremlau, A, de Villiers, E M
Format Journal Article
LanguageEnglish
Published United States 15.11.1994
Subjects
Adolescent
Adult
Aged
Child
Child, Preschool
Cholesteatoma, Middle Ear - virology
DNA Probes, HPV
DNA, Viral - analysis
Humans
Middle Aged
Papillomaviridae - genetics
Papillomaviridae - isolation & purification
Papillomavirus Infections - virology
Tumor Virus Infections - virology
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Summary:Cholesteatoma of the middle ear is a relatively common disorder, often with severe consequences. Histologically, the aggressively growing, bone-destructing form shows papillary growth and koilocytosis, which are characteristic of papillomavirus-induced lesions. A PCR (polymerase chain reaction) method using degenerate primers for the detection of any known or as yet unknown HPV (human papillomavirus) type was applied in screening 51 biopsies from 42 patients. A resulting 36% (16/45) of the cholesteatomas were found to contain papillomavirus DNA, which hybridized under stringent conditions with an HPV-II DNA probe. In 3 cases the presence of HPV-II DNA could be confirmed by sequencing the PCR products. The mere presence of this HPV DNA does not prove an etiological role of this group of viruses in the induction of cholesteatomas. It does, however, identify another group of human proliferative lesions putatively linked to papillomavirus infections.
ISSN:0020-7136
DOI:10.1002/ijc.2910590405