Accumulation of 31-kDa glycoprotein in association with the expression of embryogenic potential by spinach callus in culture

• Published in: Physiologia plantarum Vol. 114; no. 1; pp. 109 - 115
• Main Authors: Ishizaki, T. (Hokkaido Univ., Sapporo (Japan)), Megumi, C, Komai, F, Masuda, K, Oosawa, K
• Format: Journal Article
• Language: English
• Published: Oxford, UK Munksgaard International Publishers 01.01.2002; Blackwell
• Subjects: Agronomy. Soil science and plant productions; Biological and medical sciences; CAL; CALLO; CALLUS; Economic plant physiology; EMBRIOGENESIS SOMATICA; EMBRYOGENESE SOMATIQUE; Fundamental and applied biological sciences. Psychology; GLICOPROTEINAS; GLYCOPROTEINE; GLYCOPROTEINS; In vitro culture; Plant physiology and development; SOMATIC EMBRYOGENESIS; SPINACIA OLERACEA; Tissue cultures, protoplasts; Callus; Auxin; rRNA N-glycosidase; Root; Enzyme; Glycoprotein; Gibberellin; Homology; Micropropagation; Growth regulator treatment; Spinacia oleracea; Chenopodiaceae; Somatic embryo; Dicotyledones; Glycosidases; Angiospermae; Development; Hydrolases; N-Glycosidases; Plant growth substance; Spermatophyta; Gibberellic acid; Biological accumulation
• ISSN: 0031-9317; 1399-3054
• DOI: 10.1034/j.1399-3054.2002.1140115.x

Calli grown from segments of spinach (Spinacia oleracea L.) root in the presence of gibberellic acid (GA3) plus auxin, differentiated to yield somatic embryos after transfer to a medium without growth regulators, while calli formed in the absence of GA3 failed to generate any embryos. We extracted proteins from the two types of callus and analysed them by polyacrylamide gel electrophoresis. Compared with the proteins from calli formed on medium that contained only naphthaleneacetic acid (NAA) as a growth regulator, the proteins from calli grown in the presence of GA3 included appreciably higher levels of a 31‐kDa basic protein (pI = 8.8). The protein resembled type I ribosome‐inactivating proteins (EC 3.2.2.22) in terms of molecular mass, isoelectric point, sequence of amino‐terminal amino acids and extent of glycosylation. The 31‐kDa protein was barely detectable in extracts of various tissues from seedlings. Thus, it is possible that an increase in the relative level of this protein might be associated with the expression of embryogenic potential expressed by spinach callus.