Bio-green synthesis ZnO-NPs in Brassica napus pollen extract: biosynthesis, antioxidant, cytotoxicity and pro-apoptotic properties

• Published in: IET nanobiotechnology Vol. 13; no. 5; pp. 471 - 476
• Main Authors: Shahraki, Farzaneh, Tabrizi, Masoud Homayouni, Moghaddam, Mahboobeh Nakhaei, Hajebi, Sahar
• Format: Journal Article
• Language: English
• Published: The Institution of Engineering and Technology 01.07.2019
• Subjects: antibacterial activity; antioxidant properties; biochemistry; biomedical materials; bio‐green procedure; bio‐green synthesis ZnO‐NPs; bio‐green synthesised RP‐ZnO‐NPs; breast cancer cells MDA‐MB; cancer; catalase enzyme; cellular biophysics; cytotoxic effects; enzymes; field emission scanning electron microscope; field emission scanning electron microscopy; Fourier transform infrared spectra; Fourier‐transform infrared spectroscopy; II‐VI semiconductors; molecular biophysics; nanofabrication; nanomedicine; nanoparticles; particle size; pro‐apoptotic potentials; Research Article; semiconductor growth; size 26.0 nm; synthesised ZnO‐NP; time 24.0 hour; time 48.0 hour; time 72.0 hour; toxicology; transmission electron microscope; transmission electron microscopy; ultraviolet spectra; visible spectra; wide band gap semiconductors; X‐ray diffraction; zinc compounds; zinc oxide NPs; ZnO; antibacterial activity; particle size; size 26.0 nm; cellular biophysics; breast cancer cells MDA-MB; Fourier transform infrared spectra; bio-green synthesis ZnO-NPs; time 72.0 hour; nanofabrication; time 48.0 hour; X-ray diffraction; enzymes; field emission scanning electron microscopy; Fourier-transform infrared spectroscopy; visible spectra; catalase enzyme; semiconductor growth; nanoparticles; zinc compounds; transmission electron microscopy; pro-apoptotic potentials; toxicology; bio-green procedure; nanomedicine; transmission electron microscope; synthesised ZnO-NP; antioxidant properties; time 24.0 hour; ultraviolet spectra; II-VI semiconductors; biochemistry; biomedical materials; cytotoxic effects; zinc oxide NPs; cancer; bio-green synthesised RP-ZnO-NPs; ZnO; wide band gap semiconductors; molecular biophysics; field emission scanning electron microscope
• ISSN: 1751-8741; 1751-875X; 1751-875X
• DOI: 10.1049/iet-nbt.2018.5164

The bio-green methods of synthesis nanoparticles (NPs) have advantages over chemo-physical procedures due to cost-effective and ecofriendly products. The goal of current investigation is biosynthesis of zinc oxide NPs (ZnO-NPs) and evaluation of their biological assessment. Water extract of Brassica napus pollen [rapeseed (RP)] prepared and used for the synthesis of ZnO-NPs and synthesised ZnO-NP characterised using ultraviolet–visible, X-ray diffraction, Fourier-transform infrared spectroscopy, field emission scanning electron microscope and transmission electron microscope. Antioxidant properties of ZnO-NPs, cytotoxic and pro-apoptotic potentials of NPs were also evaluated. The results showed that ZnO-NPs have a hexagonal shape with 26 nm size. ZnO-NPs synthesised in RP (RP/ZnO-NPs) exhibited the good antioxidant potential compared with the butylated hydroxyanisole as a positive control. These NPs showed the cytotoxic effects against breast cancer cells (M.D. Anderson-Metastasis Breast cancer (MDA-MB)) with IC50 about 1, 6 and 6 μg/ml after 24, 48 and 72 h of exposure, respectively. RP/ZnO-NPs were found effective in increasing the expression of catalase enzyme, the enzyme involved in antioxidants properties of the cells. Bio-green synthesised RP/ZnO-NPs showed antioxidant and cytotoxic properties. The results of the present study support the advantages of using the bio-green procedure for the synthesis of NPs as an antioxidant and as anti-cancer agents.