Preparation of acetylated chitosan sponges (chitin sponges)

• Published in: Journal of applied polymer science Vol. 117; no. 3; pp. 1618 - 1623
• Main Authors: Ko, Jung A, Kim, Bum Keun, Park, Hyun Jin
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.08.2010; Wiley
• Subjects: Acetylation; Applied sciences; Biological and medical sciences; Chitin; Chitosan; degree of acetylation; Exact sciences and technology; Fibroblasts; In vitro testing; Medical sciences; Natural polymers; Physicochemistry of polymers; sponge; Sponges; Starch and polysaccharides; Surface chemistry; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Technology. Biomaterials. Equipments; Uptakes; wound healing; Cell proliferation; Biological properties; wound healing; Molecular structure; Biodegradability; Experimental study; degree of acetylation; In vitro; chitin; Dressing; Chemical modification; Chitosan derivatives; Morphology; Preparation; Biocompatibility; Oside polymer; Water absorption; Biomaterial; Sponge; Property structure relationship; Acetylation; Chitosan; Fibroblast
• ISSN: 0021-8995; 1097-4628; 1097-4628
• DOI: 10.1002/app.32053

Acetylated chitosan sponges (chitin sponges) were prepared according to acetylation time (25, 50, 75, and 100 h). As the acetylation time increased, the degree of acetylation increased, and a 75-h acetylation time produced the highest degree of acetylation (DA). The surface morphologies of samples were examined by scanning electron microscopy. Sponge samples were shown by a water uptake ability test to have higher water absorption abilities. An in vitro biodegradation test showed that sponges with a higher DA were more susceptible to lysozyme hydrolysis. Acetylated chitosan sponges were further shown by an in vitro fibroblast proliferation test to have a higher degree of cell viability on increasing the DA, with 75 h exhibiting the maximum effect. The results showed that the wound healing effect of chitosan sponges can be controlled by the DA.