The heterogeneity of the reticulum of rat peripheral lymphoid organs identified by monoclonal antibodies

We have developed a panel of six monoclonal antibodies, ED10-ED15, directed against reticular cells in peripheral lymphoid organs. Immunohistochemistry revealed prominent differences between these antibodies with regard to their tissue distribution in lymphoid and non-lymphoid organs. Furthermore, t...

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Bibliographic Details
Published inEuropean journal of immunology Vol. 19; no. 9; p. 1747
Main Authors Van den Berg, T K, Döpp, E A, Brevé, J J, Kraal, G, Dijkstra, C D
Format Journal Article
LanguageEnglish
Published Germany 01.09.1989
Subjects
Age Factors
Animals
Antibodies, Monoclonal - immunology
Fibronectins - immunology
Immunoenzyme Techniques
Laminin - immunology
Lymphoid Tissue - cytology
Lymphoid Tissue - growth & development
Lymphoid Tissue - immunology
Rats
Rats, Inbred Strains
Rats, Nude - immunology
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Summary:We have developed a panel of six monoclonal antibodies, ED10-ED15, directed against reticular cells in peripheral lymphoid organs. Immunohistochemistry revealed prominent differences between these antibodies with regard to their tissue distribution in lymphoid and non-lymphoid organs. Furthermore, the determinants recognized by ED10-ED13 were found to be differentially expressed by reticular cells occupying the various specialized compartments present in peripheral lymphoid organs. The reactivity patterns of these antibodies observed during the ontogenetic development of the spleen suggest that they recognize differentiation antigens expressed by reticular cells. In contrast, ED14 and ED15 were found to have a relatively ubiquitous tissue distribution recognizing reticular cells in each compartment, with a constitutive reactivity during splenic ontogeny. The present results indicate that reticular cells form a heterogeneous population within the lymphoid organs.
ISSN:0014-2980
DOI:10.1002/eji.1830190933