Characterization of the porcine transferrin gene (TF ) and its association with disease severity following an experimental Actinobacillus pleuropneumoniae infection

Summary Transferrin (TF)‐mediated provision of iron is essential for a productive infection by many bacterial pathogens, and iron‐depletion of TF is a first line defence against bacterial infections. Therefore, the transferrin (TF) gene can be considered a candidate gene for disease resistance. We o...

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Published inAnimal genetics Vol. 41; no. 4; pp. 424 - 427
Main Authors Daniłowicz, E., Martinez-Arias, R., Dolf, G., Singh, M., Probst, I., Tümmler, B., Höltig, D., Waldmann, K.-H., Gerlach, G.-F., Stanke, F., Leeb, T.
Format Journal Article
LanguageEnglish
Published Oxford, UK Blackwell Publishing Ltd 01.08.2010
Wiley Subscription Services, Inc
Subjects
Actinobacillus Infections - genetics
Actinobacillus Infections - microbiology
Actinobacillus Infections - pathology
Actinobacillus pleuropneumoniae
Actinobacillus pleuropneumoniae - physiology
Alternative Splicing
Animals
APP
association
Bacterial infections
Biomarkers - metabolism
Disease Models, Animal
disease resistance
Genes
Infections
Molecular Sequence Data
pig
Polymorphism, Genetic
Swine
transferrin
Transferrin - genetics
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Summary:Summary Transferrin (TF)‐mediated provision of iron is essential for a productive infection by many bacterial pathogens, and iron‐depletion of TF is a first line defence against bacterial infections. Therefore, the transferrin (TF) gene can be considered a candidate gene for disease resistance. We obtained the complete DNA sequence of the porcine TF gene, which spans 40 kb and contains 17 exons. We identified polymorphisms on a panel of 10 different pig breeds. Comparative intra‐ and interbreed sequence analysis revealed 62 polymorphisms in the TF gene including one microsatellite. Ten polymorphisms were located in the coding sequence of the TF gene. Four SNPs (c.902A>T, c.980G>A, c.1417A>G, c.1810A>C) were predicted to cause amino acid exchanges (p.Lys301Ile, p.Arg327Lys, p.Lys473Glu, p.Asn604His). We performed association analyses using six selected TF markers and 116 pigs experimentally infected with Actinobacillus pleuropneumoniae serotype 7. The analysis showed breed‐specific TF allele frequencies. In German Landrace, we found evidence for a possible association of the severity of A. pleuropneumoniae infection with TF genotypes.
Bibliography:ArticleID:AGE2012
istex:3542BC8CA2C5D4DE699703708C270A035B9F56B8
ark:/67375/WNG-1K8KCBS7-D
Present address: Department of Molecular Parasitology, Institute of Biology, Humboldt‐University Berlin, Philippstrasse 13, 10115 Berlin, Germany.
AM950267
IRAS members are: University of Veterinary Medicine Hannover, Hannover Medical School, Helmholtz Centre for Infection Research Braunschweig, Max Planck Institute for Molecular Genetics, Otto‐von‐Guericke University Magdeburg, IVD‐GmbH Hannover, LIONEX GmbH Braunschweig, RZPD GmbH Berlin, and the contributing scientists (in alphabetical order) Benga L, Blöcker H, Daniłowicz E, Drungowski M, Gerlach GF, Herwig R, Höltig D, Kahlisch D, Leeb T, Martinez‐Arias R, Naim HY, Pabst R, Probst I, Radelof U, Rehm T, Rothkötter HJ, Singh M, Spalleck R, Stanke F, Strutzberg‐Minder K, Thies K, Tümmler B, Valentin‐Weigand P, Wagner F, Waldmann KH.
The nucleotide sequence has been submitted to the EMBL nucleotide sequence database under the accession number
.
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0268-9146
1365-2052
DOI:10.1111/j.1365-2052.2009.02012.x