Drosophila laminin: sequence of B2 subunit and expression of all three subunits during embryogenesis

• Published in: The Journal of cell biology Vol. 109; no. 5; pp. 2441 - 2453
• Main Authors: Montell, D.J. (Carnegie Institution of Washington, Baltimore, MD), Goodman, C.S
• Format: Journal Article
• Language: English
• Published: New York, NY Rockefeller University Press 01.11.1989; The Rockefeller University Press
• Subjects: ACIDE AMINE; Amino Acid Sequence; AMINO ACIDS; AMINOACIDOS; Analytical, structural and metabolic biochemistry; Animals; Axons; Base Sequence; Biological and medical sciences; Central nervous system; Cloning, Molecular; Complementary DNA; DESARROLLO EMBRIONARIO; DEVELOPPEMENT EMBRYONNAIRE; Diptera; DNA - genetics; DROSOPHILA; Drosophila - embryology; Drosophila - genetics; Drosophilidae; Embryo, Nonmammalian - metabolism; embryogenesis; EMBRYONIC DEVELOPMENT; Embryos; Fundamental and applied biological sciences. Psychology; Gene Expression; Genes; Glycoproteins; Laminin - genetics; Macromolecular Substances; Mesoderm; Messenger RNA; Mice; Molecular Sequence Data; Neuroglia; Neurons; Nucleic Acid Hybridization; NUCLEOTIDE; NUCLEOTIDES; NUCLEOTIDOS; PROTEINAS; PROTEINE; PROTEINS; Sequence Homology, Nucleic Acid; Species Specificity; Peripheral nervous system; Embryonic development; Molecular hybridization; Nucleotide sequence; Immunocytochemistry; Insecta; In situ; Central nervous system; Glycoproteins; Gene expression; Subunit; Drosophilidae; Northern blotting; Autoradiography; Laminin; Complementary DNA; Arthropoda; Molecular probe; Invertebrata; Localization; Drosophila melanogaster; Diptera; Aminoacid sequence
• ISSN: 0021-9525; 1540-8140
• DOI: 10.1083/jcb.109.5.2441

In a previous study, we described the cloning of the genes encoding the three subunits of Drosophila laminin, a substrate adhesion molecule, and the cDNA sequence of the B1 subunit (Montell and Goodman, 1988). This analysis revealed the similarity of Drosophila laminin with the mouse and human complexes in subunit composition, domain structure, and amino acid sequence. In this paper, we report the deduced amino acid sequence of the B2 subunit. We then describe the expression and tissue distribution of the three subunits of laminin during Drosophila embryogenesis using both in situ hybridization and immunolocalization techniques, with particular emphasis on its expression in and around the developing nervous system.