Induction of expression of protein disulphide-isomerase during lymphocyte maturation stimulated by bacterial lipopolysaccharide

Protein disulphide-isomerase (PDI) activity, and the level of immunodetectable PDI protein, were monitored in splenic lymphocytes and in BCL 1 cells during culture in the presence of various activating factors. Bacterial lipopolysaccharide stimulated induction of PDI in splenic B cells and BCL 1 cel...

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Bibliographic Details
Published inFEBS letters Vol. 242; no. 2; pp. 357 - 362
Main Authors Paver, Jan L., Freedman, Robert B., Michael, R., Parkhouse, E.
Format Journal Article
LanguageEnglish
Published England Elsevier B.V 02.01.1989
Subjects
Animals
B-Lymphocytes - cytology
B-Lymphocytes - enzymology
Cell Differentiation
Endoplasmic reticulum
Enzyme Induction
In Vitro Techniques
Isomerases - biosynthesis
Lipopolysaccharides - pharmacology
Lymphocyte Activation
Lymphocyte maturation
Mice
Plasma Cells - cytology
Plasma Cells - enzymology
Protein disulfide-isomerase
Protein Disulfide-Isomerases
Time Factors
Lymphocyte maturation
Protein disulfide-isomerase
Endoplasmic reticulum
Enzyme induction
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Summary:Protein disulphide-isomerase (PDI) activity, and the level of immunodetectable PDI protein, were monitored in splenic lymphocytes and in BCL 1 cells during culture in the presence of various activating factors. Bacterial lipopolysaccharide stimulated induction of PDI in splenic B cells and BCL 1 cells. The time-course and specificity of induction indicated that the increase in expression of PDI is closely coupled to the final stages of B cell differentiation into antibody-producing plasma cells. The system will prove valuable in studies on the control of expression of PDI.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(89)80501-0