Tissue expression and the host's immunological recognition of a Rhipicephalus microplus paramyosin

• Published in: Veterinary parasitology Vol. 197; no. 1-2; pp. 304 - 311
• Main Authors: Leal, Bruna F., Seixas, Adriana, Mattos, Roberta T., Coutinho, Mariana L., Masuda, Aoi, da Silva Vaz, Itabajara, Ferreira, Carlos A.S.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 18.10.2013
• Subjects: adults; Animals; Antibodies - blood; Antigens - immunology; Antigens - metabolism; Boophilus microplus; Bos taurus; Cattle; Cattle Diseases - blood; Cattle Diseases - immunology; chemical control; eggs; enzyme-linked immunosorbent assay; fat body; Female; females; gene expression; Gene Expression Regulation - immunology; Haematophagous; herds; immune system; Ixodidae; Larva - metabolism; larvae; Male; Naturally acquired tick resistance; Ovum - metabolism; Paramyosin; parasites; Rhipicephalus; Rhipicephalus (Boophilus) microplus; Rhipicephalus - immunology; Rhipicephalus - metabolism; salivary glands; tick infestations; Tick-bovine relationship; Tropomyosin - immunology; Tick-bovine relationship; Haematophagous; Paramyosin; Rhipicephalus (Boophilus) microplus; Naturally acquired tick resistance
• ISSN: 0304-4017; 1873-2550
• DOI: 10.1016/j.vetpar.2013.06.020

Rhipicephalus microplus is a parasite that causes economic losses in cattle herds, and immunological control is the most promising alternative to replace chemical control. The muscular protein paramyosin has been additionally found in non-muscle tissues and characterized as presenting activities that enable the evasion of the host's immune system in various parasites. This report investigated the recognition level of paramyosin by sera of infested bovines, its expression in tissues, organs and different life stages of R. microplus. ELISA analyses showed that paramyosin and salivary gland extract were recognized by infested Bos taurus and B. indicus sera. Paramyosin gene expression was evaluated in egg, larvae, adult male, and several tissues of partially- and fully-engorged females by qRT-PCR, showing the highest expression levels in fat body. These results show that R. microplus paramyosin is immunologically recognized during the tick infestation and together with the high transcription rate found in organs that do not present a highly developed musculature, further suggests that it may possess additional, non-muscle functions in the tick-bovine relationship.