Development of a microalgal PAM test method for Cu(II) in waters: comparison of using spectrofluorometry
Test methods are needed to monitor Cu concentrations in reservoirs and water supplies. Dictyosphaerium chlorelloides (Chlorophyta) cells were immobilized in a silicate sol-gel and the toxic effects of Cu(II) were examined using different techniques: fluorescence measurements (using a spectrofluorome...
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Published in | Ecotoxicology (London) Vol. 19; no. 6; pp. 1059 - 1065 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Boston
Boston : Springer US
01.08.2010
Springer US Springer Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | Test methods are needed to monitor Cu concentrations in reservoirs and water supplies. Dictyosphaerium chlorelloides (Chlorophyta) cells were immobilized in a silicate sol-gel and the toxic effects of Cu(II) were examined using different techniques: fluorescence measurements (using a spectrofluorometer with an optic fiber coupled to a flow cell or a 96-well-plate reader) or by Pulse Amplitude Modulation (PAM) parameters using a portable instrument and the pulse saturation method. Fm′ and qN were the most sensitive indicator parameters when performing Cu analysis in water. D. chlorelloides PAM biosensor presented a detection limit of 0.6 mg l⁻¹ for Cu(II), within the limits to establish if Cu concentrations exceeded regulatory levels. Moreover, a 1.9 mg Cu l⁻¹ (30 μM) resistant strain of the D. chlorelloides microalgae was produced in order to obtain more selectivity on the metal determination. |
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Bibliography: | http://dx.doi.org/10.1007/s10646-010-0487-y ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0963-9292 1573-3017 |
DOI: | 10.1007/s10646-010-0487-y |