A Novel BODIPY Quaternary Ammonium Salt-Based Fluorescent Probe: Synthesis, Physical Properties, and Live-Cell Imaging

The development of biological fluorescent probes is of great significance to the field of cancer bio-imaging. However, most current probes within the bulky hydrophobic group have limited application in aqueous medium and restricted imaging under physiological conditions. Herein, we proposed two effi...

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Bibliographic Details
Published inFrontiers in chemistry Vol. 9; p. 650006
Main Authors Deng, Peng, Xiao, Fuyan, Wang, Zhou, Jin, Guofan
Format Journal Article
LanguageEnglish
Published Switzerland Frontiers Media S.A 12.03.2021
Subjects
BODIPY
Chemistry
HeLa cells
in vitro imaging
in vivo imaging
quaternary ammonium salt
HeLa cells
in vitro imaging
BODIPY
in vivo imaging
quaternary ammonium salt
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Summary:The development of biological fluorescent probes is of great significance to the field of cancer bio-imaging. However, most current probes within the bulky hydrophobic group have limited application in aqueous medium and restricted imaging under physiological conditions. Herein, we proposed two efficient molecules to study their physical properties and imaging work, and the absorption and fluorescence intensity were collected with varying ions attending in aqueous medium. We enhance the water solubility through the quaternization reaction and form a balance between hydrophilic and hydrophobicity with dipyrrome-theneboron difluoride (BODIPY) fluorophore. We introduced pyridine and dimethylaminopyridine (DMAP) by quaternization and connected the BODIPY fluorophore by ethylenediamine. The final synthesized probes have achieved ideal affinity with HeLa cells (human cervical carcinoma cell line) in live-cell imaging which could be observed by Confocal Microscope. The probes also have a good affinity with subcutaneous tumor cells in mice in imaging, which may make them candidates as oncology imaging probes.
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Hanjun Cheng, Institute for Systems Biology (ISB), United States
Reviewed by: Zhonghan Li, Oregon Health and Science University, United States
Edited by: Min Xue, University of California, Riverside, United States
This article was submitted to Analytical Chemistry, a section of the journal Frontiers in Chemistry
ISSN:2296-2646
2296-2646
DOI:10.3389/fchem.2021.650006