Activin/Nodal/TGF-β Pathway Inhibitor Accelerates BMP4-Induced Cochlear Gap Junction Formation During in vitro Differentiation of Embryonic Stem Cells

• Published in: Frontiers in cell and developmental biology Vol. 9; p. 602197
• Main Authors: Fukunaga, Ichiro, Oe, Yoko, Chen, Cheng, Danzaki, Keiko, Ohta, Sayaka, Koike, Akito, Ikeda, Katsuhisa, Kamiya, Kazusaku
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 21.04.2021
• Subjects: Cell and Developmental Biology; cochlear supporting cells; connexin 26 and 30; embryonic stem cells; gap junction beta 2 gene; gap junction plaques; SB431542; cochlear supporting cells; embryonic stem cells; gap junction beta 2 gene; gap junction plaques; SB431542; connexin 26 and 30
• ISSN: 2296-634X; 2296-634X
• DOI: 10.3389/fcell.2021.602197

Mutations in gap junction beta-2 ( ), the gene that encodes connexin 26 (CX26), are the most frequent cause of hereditary deafness worldwide. We recently developed an model of 2-related deafness (induced CX26 gap junction-forming cells; iCX26GJCs) from mouse induced pluripotent stem cells (iPSCs) by using Bone morphogenetic protein 4 (BMP4) signaling-based floating cultures (serum-free culture of embryoid body-like aggregates with quick aggregation cultures; hereafter, SFEBq cultures) and adherent cultures. However, to use these cells as a disease model platform for high-throughput drug screening or regenerative therapy, cell yields must be substantially increased. In addition to BMP4, other factors may also induce CX26 gap junction formation. In the SFEBq cultures, the combination of BMP4 and the Activin/Nodal/TGF-β pathway inhibitor SB431542 (SB) resulted in greater production of isolatable CX26-expressing cell mass (CX26 vesicles) and higher mRNA levels than BMP4 treatment alone, suggesting that SB may promote BMP4-mediated production of CX26 vesicles in a dose-dependent manner, thereby increasing the yield of highly purified iCX26GJCs. This is the first study to demonstrate that SB accelerates BMP4-induced iCX26GJC differentiation during stem cell floating culture. By controlling the concentration of SB supplementation in combination with CX26 vesicle purification, large-scale production of highly purified iCX26GJCs suitable for high-throughput drug screening or regenerative therapy for -related deafness may be possible.