Their C-termini divide Brassica rapa FT-like proteins into FD-interacting and FD-independent proteins that have different effects on the floral transition
Members of the FLOWERING LOCUS T (FT)-like clade of phosphatidylethanolamine-binding proteins (PEBPs) induce flowering by associating with the basic leucine zipper (bZIP) transcription factor FD and forming regulatory complexes in angiosperm species. However, the molecular mechanism of the FT-FD het...
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Published in | Frontiers in plant science Vol. 13; p. 1091563 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
Frontiers Media S.A
12.01.2023
|
Subjects | |
Online Access | Get full text |
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Summary: | Members of the FLOWERING LOCUS T (FT)-like clade of phosphatidylethanolamine-binding proteins (PEBPs) induce flowering by associating with the basic leucine zipper (bZIP) transcription factor FD and forming regulatory complexes in angiosperm species. However, the molecular mechanism of the FT-FD heterocomplex in Chinese cabbage (
ssp.
) is unknown. In this study, we identified 12
genes and focused our functional analysis on four
genes by overexpressing them individually in an
loss-of-function mutant in
. We determined that
and
promote flowering by upregulating the expression of floral meristem identity genes, whereas
and
, although close in sequence to their Arabidopsis counterparts, had no clear effect on flowering in either long- or short-day photoperiods. We also simultaneously genetically inactivated
and
in Chinese cabbage using CRISPR/Cas9-mediated genome editing, which revealed that
and
may play key roles in inflorescence organogenesis as well as in the transition to flowering. We show that BrFT-like proteins, except for BrTSF, are functionally divided into FD interactors and non-interactors based on the presence of three specific amino acids in their C termini, as evidenced by the observed interconversion when these amino acids are mutated. Overall, this study reveals that although
homologs are conserved, they may have evolved to exert functionally diverse functions in flowering
their potential to be associated with FD or independently from FD in
. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Edited by: Goetz Hensel, Heinrich Heine University Düsseldorf, Germany Reviewed by: Rainer Melzer, University College Dublin, Ireland; Francesca Bellinazzo, Wageningen University and Research, Netherlands This article was submitted to Plant Biotechnology, a section of the journal Frontiers in Plant Science These authors have contributed equally to this work |
ISSN: | 1664-462X 1664-462X |
DOI: | 10.3389/fpls.2022.1091563 |