Crystallization and Preliminary X-ray Diffraction Studies of Bacillus stearothermophilus Farnesyl Diphosphate Synthase Expressed in Escherichia coli

Thermostable farnesyl diphosphate synthase (EC 2.5.1.10) from Bacillus stearothermophilus, which was overexpressed in Escherichia coli, has been crystallized by the vapor-diffusion procedure. Tetragonal crystals were obtained using ammonium sulfate as a precipitant. The crystals diffracted X-rays to...

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Published inJournal of molecular biology Vol. 233; no. 4; pp. 787 - 788
Main Authors Nakane, Hiroyuki, Koyama, Tanetoshi, Obata, Shusei, Osabe, Masami, Takeshita, Ayumi, Nishino, Tokuzo, Ogura, Kyozo, Miki, Kunio
Format Journal Article
LanguageEnglish
Published Oxford Elsevier Ltd 20.10.1993
Elsevier
Subjects
Alkyl and Aryl Transferases
Analytical, structural and metabolic biochemistry
Bacillus stearothermophilus
Biological and medical sciences
Cloning, Molecular
Crystalline structure
Crystallization
Enzymes and enzyme inhibitors
Escherichia coli
farnesyl diphosphate synthase
Fundamental and applied biological sciences. Psychology
Geobacillus stearothermophilus - chemistry
Geobacillus stearothermophilus - enzymology
Geobacillus stearothermophilus - genetics
Geranyltranstransferase
Molecular biophysics
prenyltransferase
Structure in molecular biology
thermostable enzyme
Transferases
Transferases - biosynthesis
Transferases - chemistry
Transferases - genetics
X-Ray Diffraction
crystallization
X-ray diffraction
farnesyl diphosphate synthase
prenyltransferase
thermostable enzyme
Bacillales
Enzyme
Transferases
Bacillus stearothermophilus
Geranyltranstransferase
Bacteria
Bacillaceae
Lattice parameters
Structural analysis
Crystalline structure
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Summary:Thermostable farnesyl diphosphate synthase (EC 2.5.1.10) from Bacillus stearothermophilus, which was overexpressed in Escherichia coli, has been crystallized by the vapor-diffusion procedure. Tetragonal crystals were obtained using ammonium sulfate as a precipitant. The crystals diffracted X-rays to about 3 Å resolution. The diffraction pattern indicated that the space group is I4122 with unit-cell dimensions of a = b = 114 Å and c = 247 Å. It is thought that the asymmetric unit comprises two or three molecules of farnesyl diphosphate synthase.
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ISSN:0022-2836
1089-8638
DOI:10.1006/jmbi.1993.1554