Necator americanus Ancylostoma Secreted Protein-2 ( Na -ASP-2) Binds an Ascaroside (ascr#3) in Its Fatty Acid Binding Site
During their infective stages, hookworms release excretory-secretory (E-S) products, small molecules, and proteins to help evade and suppress the host's immune system. Small molecules found in E-S products of mammalian hookworms include nematode derived metabolites like ascarosides, which are c...
Saved in:
Published in | Frontiers in chemistry Vol. 8; p. 608296 |
---|---|
Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
Frontiers Media S.A
17.12.2020
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | During their infective stages, hookworms release excretory-secretory (E-S) products, small molecules, and proteins to help evade and suppress the host's immune system. Small molecules found in E-S products of mammalian hookworms include nematode derived metabolites like ascarosides, which are composed of the sugar ascarylose linked to a fatty acid side chain. The most abundant proteins found in hookworm E-S products are members of the protein family known as
secreted protein (ASP). In this study, two ascarosides and their fatty acid moieties were synthesized and tested for
binding to
-ASP-2 using both a ligand competition assay and microscale thermophoresis. The fatty acid moieties of both ascarosides tested and ascr#3, an ascaroside found in rat hookworm E-S products, bind to
palmitate binding cavity. These molecules were confirmed to bind to the palmitate but not the sterol binding sites. An ascaroside, oscr#10, which is not found in hookworm E-S products, does not bind to
-ASP-2. More studies are required to determine the structural basis of ascarosides binding by
-ASP-2 and to understand the physiological significance of these observations. |
---|---|
Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Reviewed by: Paul Giacomin, James Cook University, Australia; Norelle Daly, James Cook University, Australia; Lisa Harrison, Yale University, United States Edited by: Julio A. Camarero, University of Southern California, Los Angeles, United States This article was submitted to Chemical Biology, a section of the journal Frontiers in Chemistry These authors have contributed equally to this work |
ISSN: | 2296-2646 2296-2646 |
DOI: | 10.3389/fchem.2020.608296 |