Extracellular Vesicles From Liver Progenitor Cells Downregulates Fibroblast Metabolic Activity and Increase the Expression of Immune-Response Related Molecules
Extracellular vesicles (EVs) mediate cell-to-cell crosstalk whose content can induce changes in acceptor cells and their microenvironment. MLP29 cells are mouse liver progenitor cells that release EVs loaded with signaling cues that could affect cell fate. In the current work, we incubated 3T3-L1 mo...
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Published in | Frontiers in cell and developmental biology Vol. 8; p. 613583 |
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Main Authors | , , , , , , , , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
Frontiers Media S.A
12.01.2021
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Subjects | |
Online Access | Get full text |
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Summary: | Extracellular vesicles (EVs) mediate cell-to-cell crosstalk whose content can induce changes in acceptor cells and their microenvironment. MLP29 cells are mouse liver progenitor cells that release EVs loaded with signaling cues that could affect cell fate. In the current work, we incubated 3T3-L1 mouse fibroblasts with MLP29-derived EVs, and then analyzed changes by proteomics and transcriptomics. Results showed a general downregulation of protein and transcript expression related to proliferative and metabolic routes dependent on TGF-beta. We also observed an increase in the ERBB2 interacting protein (ERBIN) and Cxcl2, together with an induction of ribosome biogenesis and interferon-related response molecules, suggesting the activation of immune system signaling. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Present address: Jose L. Lavin, Applied Mathematics Department, Bioinformatics Unit NEIKER-(BRTA), Bizkaia Technology Park, Bizkaia, Spain; Marc Clos-Garcia, Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark This article was submitted to Membrane Traffic, a section of the journal Frontiers in Cell and Developmental Biology Edited by: Anna Onnis, University of Siena, Italy Reviewed by: Alexander Grigoryevich Tonevitsky, National Research University Higher School of Economics, Russia; Olof Gidlöf, Lund University, Sweden |
ISSN: | 2296-634X 2296-634X |
DOI: | 10.3389/fcell.2020.613583 |